Characterization of IS1676 from Rhodococcus erythropolis SQ1.

TitleCharacterization of IS1676 from Rhodococcus erythropolis SQ1.
Publication TypeJournal Article
Year of Publication1999
AuthorsLessard, PA, O'Brien, XM, Ahlgren, NA, Ribich, SA, Sinskey, AJ
JournalAppl Microbiol Biotechnol
Date Published1999 Nov
KeywordsBase Sequence, DNA Transposable Elements, Genome, Bacterial, Molecular Sequence Data, Mycobacterium, Open Reading Frames, Rhodococcus, Sequence Alignment, Sequence Homology, Nucleic Acid

To develop a transposable element-based system for mutagenesis in Rhodococcus, we used the sacB gene from Bacillus subtilis to isolate a novel transposable element, IS1676, from R. erythropolis SQ1. This 1693 bp insertion sequence is bounded by imperfect (10 out of 13 bp) inverted repeats and it creates 4 bp direct repeats upon insertion. Comparison of multiple insertion sites reveals a preference for the sequence 5'-(C/T)TA(A/G)-3' in the target site. IS1676 contains a single, large (1446 bp) open reading frame with coding potential for a protein of 482 amino acids. IS1676 may be similar to an ancestral transposable element that gave rise to repetitive sequences identified in clinical isolates of Mycobacterium kansasii. Derivatives of IS1676 should be useful for analysis of Rhodococcus strains, for which few other genetic tools are currently available.

Alternate JournalAppl Microbiol Biotechnol
Citation Key222
PubMed ID10616714