Direct evidence for a xylose metabolic pathway in Saccharomyces cerevisiae.

TitleDirect evidence for a xylose metabolic pathway in Saccharomyces cerevisiae.
Publication TypeJournal Article
Year of Publication1986
AuthorsBatt, CA, Caryallo, S, Easson, DD, Akedo, M, Sinskey, AJ
JournalBiotechnol Bioeng
Volume28
Issue4
Pagination549-53
Date Published1986 Apr
ISSN0006-3592
Abstract

Xylose transport, xylose reductase, and xylitol dehydrogenase activities are demonstrated in Saccharomyces cerevisiae. The enzymes in the xylose catabolic pathway necessary for the conversion of xylose to xylulose are present, although S. cerevisiae cannot grow on xylose as a sole carbon source. Xylose transport is less efficient than glucose transport, and its rate is dependent upon aeration. Xylose reductase appears to be a xylose inducible enzyme and xylitol dehydrogenase activity is constitutive, although both are repressed by glucose. Both xylose reductase and xylitol dehydrogenase activities are five- to tenfold lower in S. cerevisiae as compared to Candida utilis. In vivo conversion of (14)C-xylose in S. cerevisiae is demonstrated and xylitol is detected, although no significant levels of any other (14)C-labeled metabolites (e. g., ethanol) are observed.

DOI10.1002/bit.260280411
Alternate JournalBiotechnol Bioeng
Citation Key256
PubMed ID18555359