Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules.

TitleImmunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules.
Publication TypeJournal Article
Year of Publication1993
AuthorsGerngross, TU, Reilly, P, Stubbe, J, Sinskey, AJ, Peoples, OP
JournalJ Bacteriol
Volume175
Issue16
Pagination5289-93
Date Published1993 Aug
ISSN0021-9193
KeywordsAcyltransferases, Alcaligenes, Cell Compartmentation, Cytoplasmic Granules, Immunohistochemistry, Models, Biological, Viral Proteins
Abstract

Antibodies raised against the Alcaligenes eutrophus poly-beta-hydroxybutyrate (PHB) synthase polypeptide were used for immunocytochemical localization of the synthase enzyme in whole cells and purified PHB granules. The data presented demonstrate for the first time that the synthase enzyme is located on the surface of the PHB granule rather than being incorporated inside the granule during its formation. From these basic observations and data from the recent literature, a model of granule assembly is proposed.
DOI10.1128/jb.175.16.5289-5293.1993
Alternate JournalJ Bacteriol
Citation Key184
PubMed ID8349571
PubMed Central IDPMC205001
Grant List1 S10 RR05734-01 / RR / NCRR NIH HHS / United States