Improved detergent-based recovery of polyhydroxyalkanoates (PHAs).

TitleImproved detergent-based recovery of polyhydroxyalkanoates (PHAs).
Publication TypeJournal Article
Year of Publication2011
AuthorsYang, Y-H, Brigham, C, Willis, L, Rha, CK, Sinskey, A
JournalBiotechnol Lett
Volume33
Issue5
Pagination937-42
Date Published2011 May
ISSN1573-6776
KeywordsChemical Fractionation, Cupriavidus necator, Detergents, Escherichia coli, Polyhydroxyalkanoates
Abstract

Extracting polyhydroxyalkanoate (PHA) polymer from bacterial cells often involves harsh conditions, including use of environmentally harmful solvents. We evaluated different detergents under various conditions to extract PHA from Ralstonia eutropha and Escherichia coli cells. Most detergents tested recovered highly pure PHA polymer from cells in amounts that depended on the percentage of polymer present in the cell. Detergents such as linear alkylbenzene sulfonic acid (LAS-99) produced a high yield of high purity polymer, and less detergent was needed compared to the amount of SDS to produce comparable yields. LAS-99 also has the advantage of being biodegradable and environmentally safe. Chemical extraction of PHA with detergents could potentially minimize or eliminate the need to use harsh organic solvents, thus making industrial PHA production a cleaner technology process.

DOI10.1007/s10529-010-0513-4
Alternate JournalBiotechnol Lett
Citation Key104
PubMed ID21207109