Large-scale production of mammalian cells and their products: engineering principles and barriers to scale-up.

TitleLarge-scale production of mammalian cells and their products: engineering principles and barriers to scale-up.
Publication TypeJournal Article
Year of Publication1983
AuthorsGlacken, MW, Fleischaker, RJ, Sinskey, AJ
JournalAnn N Y Acad Sci
Volume413
Pagination355-72
Date Published1983
ISSN0077-8923
KeywordsAnimals, Biomedical Engineering, Cell Adhesion, Cell Physiological Phenomena, Cells, Cultured, Culture Techniques, Kinetics
Abstract

Mammalian cell products have great medical and clinical importance, but to date, production methods employed to manufacture these products on a large scale are not as cost efficient as they could be. The implementation of process control would greatly improve the productivity of these products. Recently developed methods to produce cells on a large scale, such as microcarriers, artificial capillaries, tubular spiral film, and microencapsulation must be optimized, and the problem of oxygen transfer limitation must be solved. The accumulation of potentially toxic waste products can inhibit growth and reduce productivity. This effect can be reduced by either adjusting the environmental parameters of a fed-batch culture, so that the cell's metabolism is shifted away from producing these compounds, or by continually perfusing medium through the culture. If these technical barriers can be overcome, the cost of producing products derived from mammalian cells can be greatly reduced.

DOI10.1111/j.1749-6632.1983.tb47912.x
Alternate JournalAnn N Y Acad Sci
Citation Key243
PubMed ID6584068
Grant List5-T32-CA09258-05 / CA / NCI NIH HHS / United States