Solving Large Problems With Small Biofactories

Large-scale production of mammalian cells and their products: engineering principles and barriers to scale-up.

Title

Large-scale production of mammalian cells and their products: engineering principles and barriers to scale-up.

Publication Type
Journal Article
Year of Publication
1983
Journal
Ann N Y Acad Sci
Volume
413
Pagination
355-72
Date Published
1983
ISSN
0077-8923
Abstract

Mammalian cell products have great medical and clinical importance, but to date, production methods employed to manufacture these products on a large scale are not as cost efficient as they could be. The implementation of process control would greatly improve the productivity of these products. Recently developed methods to produce cells on a large scale, such as microcarriers, artificial capillaries, tubular spiral film, and microencapsulation must be optimized, and the problem of oxygen transfer limitation must be solved. The accumulation of potentially toxic waste products can inhibit growth and reduce productivity. This effect can be reduced by either adjusting the environmental parameters of a fed-batch culture, so that the cell’s metabolism is shifted away from producing these compounds, or by continually perfusing medium through the culture. If these technical barriers can be overcome, the cost of producing products derived from mammalian cells can be greatly reduced.

Alternate Journal
Ann N Y Acad Sci
Citation Key
243

PubMed ID

6584068

Grant List

5-T32-CA09258-05 / CA / NCI NIH HHS / United States