|Manipulation of Ralstonia eutropha carbon storage pathways to produce useful bio-based products.
|Year of Publication
|Brigham, CJ, Zhila, N, Shishatskaya, E, Volova, TG, Sinskey, AJ
|Biological Products, Biotechnology, Carbon, Cupriavidus necator, Gene Expression Regulation, Bacterial, Genetic Engineering, Metabolic Networks and Pathways, Polyhydroxyalkanoates
Ralstonia eutrophais a Gram-negative betaproteobacterium found natively in soils that can utilize a wide array of carbon sources for growth, and can store carbon intracellularly in the form of polyhydroxyalkanoate. Many aspects of R. eutrophamake it a good candidate for use in biotechnological production of polyhydroxyalkanoate and other bio-based, value added compounds. Manipulation of the organism's carbon flux is a cornerstone to success in developing it as a biotechnologically relevant organism. Here, we examine the methods of controlling and adapting the flow of carbon in R. eutrophametabolism and the wide range of compounds that can be synthesized as a result. The presence of many different carbon utilization pathways and the custom genetic toolkit for manipulation of those pathways gives R. eutrophaa versatility that allows it to be a biotechnologically important organism.