Molecular cloning, nucleotide sequence and fine-structural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-1,6-biphosphate aldolase to class I and class II aldolases.

TitleMolecular cloning, nucleotide sequence and fine-structural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-1,6-biphosphate aldolase to class I and class II aldolases.
Publication TypeJournal Article
Year of Publication1989
Authorsvon der Osten, CH, Barbas, CF, Wong, CH, Sinskey, AJ
JournalMol Microbiol
Volume3
Issue11
Pagination1625-37
Date Published1989 Nov
ISSN0950-382X
KeywordsAmino Acid Sequence, Base Sequence, Cloning, Molecular, Corynebacterium, DNA, Bacterial, Fructose-Bisphosphate Aldolase, Genes, Bacterial, Glucose, Molecular Sequence Data, Restriction Mapping, Transcription, Genetic
Abstract

The Corynebacterium glutamicum fda gene encoding fructose-1,6-biphosphate (FBP) aldolase has been isolated by complementation of an Escherichia coli mutant. The nucleotide sequence of a 3371 bp chromosomal fragment containing the C. glutamicum fda gene was determined. The N-terminal amino acid sequence of C. glutamicum FBP aldolase identified the correct initiation site for the fda gene, and a molecular weight of 37,092 was predicted for the fda polypeptide. S1 nuclease mapping identified the transcriptional start site, and Northern hybridization analysis indicated that the fda gene encodes a single 1.3 kb transcript. The primary structure of C. glutamicum FBP aldolase shows strong homology to class II FBP aldolases. Conservation of primary structure was observed between class I and class II aldolases, but several residues essential for catalytic activity in class I aldolases were absent from class II aldolases.

DOI10.1111/j.1365-2958.1989.tb00148.x
Alternate JournalMol Microbiol
Citation Key152
PubMed ID2615658