|Title||The molecular structure of the Corynebacterium glutamicum threonine synthase gene.|
|Publication Type||Journal Article|
|Year of Publication||1990|
|Authors||Han, KS, Archer, JA, Sinskey, AJ|
|Date Published||1990 Oct|
|Keywords||Alleles, Amino Acid Sequence, Bacillus subtilis, Blotting, Northern, Carbon-Oxygen Lyases, Corynebacterium, Escherichia coli, Genes, Bacterial, Lyases, Molecular Sequence Data, Molecular Structure, Plasmids, Protein Biosynthesis, RNA, Messenger, Sequence Homology, Nucleic Acid, Transcription, Genetic|
The minimal region encoding the Corynebacterium glutamicum threonine synthase structural gene and its promoter was mapped by deletion analysis and complementation of the C. glutamicum thrC allele to a 1.6 kb region of the recombinant plasmid pFS80. The nucleotide sequence of this and flanking DNA was determined. The transcription and translation start points were identified by S1 mapping analysis and amino-terminal protein sequencing, respectively. The thrC gene encodes a 54481-Dalton polypeptide product. Translation of the thrC mRNA initiates only six nucleotides downstream from transcription. The length of the mRNA transcript is consistent with a single gene transcription unit. The C. glutamicum thrC gene is expressed independently of the other threonine-specific genes hom and thrB.
|Alternate Journal||Mol Microbiol|