Nucleotide sequence of the lysA gene of Corynebacterium glutamicum and possible mechanisms for modulation of its expression.

TitleNucleotide sequence of the lysA gene of Corynebacterium glutamicum and possible mechanisms for modulation of its expression.
Publication TypeJournal Article
Year of Publication1988
AuthorsYeh, P, Sicard, AM, Sinskey, AJ
JournalMol Gen Genet
Volume212
Issue1
Pagination112-9
Date Published1988 Apr
ISSN0026-8925
KeywordsAmino Acid Sequence, Bacterial Proteins, Base Sequence, Carboxy-Lyases, Cloning, Molecular, Corynebacterium, Cosmids, DNA Restriction Enzymes, Escherichia coli, Gene Expression Regulation, Genes, Genes, Bacterial, Molecular Sequence Data, Plasmids
Abstract

Sequence analysis localized the lysA gene of Corynebacterium glutamicum strain AS019 within a 1.35 kb open reading frame, potentially encoding a 445 amino acid product. Immediately downstream from this gene we found a potential rho-independent transcription terminator, while the 5' flanking region (300 bp) harbors unusual topological and structural features, located in the vicinity of a potential ribosome binding site. Within this upstream region, enzymatic and genetic analyses indicated the occurrence of a promoter responsible for significant, although weak, expression of the encoded enzymatic activity. The same significant expression level was observed with a plasmid harboring an additional 0.5 kb of genomic information upstream from lysA, while its full expression apparently requires 2 kb of additional genomic information located immediately upstream from the cloned gene. The upstream sequence requirement apparently associated with the full expression of the lysA gene of C. glutamicum shows some similarity with the Escherichia coli system.

DOI10.1007/BF00322452
Alternate JournalMol Gen Genet
Citation Key146
PubMed ID2836698