Oxidized redox state of glutathione in the endoplasmic reticulum.

TitleOxidized redox state of glutathione in the endoplasmic reticulum.
Publication TypeJournal Article
Year of Publication1992
AuthorsHwang, C, Sinskey, AJ, Lodish, HF
Date Published1992 Sep 11
KeywordsAmino Acid Sequence, Animals, Chromatography, High Pressure Liquid, Chromatography, Thin Layer, Dogs, Endoplasmic Reticulum, Glutathione, Glutathione Disulfide, Kinetics, Microsomes, Models, Biological, Molecular Sequence Data, Oligopeptides, Oxidation-Reduction, Pancreas

The redox state of the endoplasmic reticulum (ER) was measured with the peptide N-Acetyl-Asn-Tyr-Thr-Cys-NH2. The peptide diffused across cellular membranes; some became glycosylated and thus trapped within the secretory pathway, and its cysteine residue underwent reversible thiol-disulfide exchanges with the surrounding redox buffer. Glycosylated peptides from cells were disulfide-linked to glutathione, indicating that glutathione is the major redox buffer in the secretory pathway. The redox state of the secretory pathway was more oxidative than that of the cytosol; the ratio of reduced glutathione to the disulfide form (GSH/GSSG) within the secretory pathway ranged from 1:1 to 3:1, whereas the overall cellular GSH/GSSG ratio ranged from 30:1 to 100:1. Cytosolic glutathione was also transported into the lumen of microsomes in a cell-free system. Although how the ER maintains an oxidative environment is not known, these results suggest that the demonstrated preferential transport of GSSG compared to GSH into the ER lumen may contribute to this redox compartmentation.

Alternate JournalScience
Citation Key120
PubMed ID1523409