| Title | Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). |
| Publication Type | Journal Article |
| Year of Publication | 1989 |
| Authors | Peoples, OP, Sinskey, AJ |
| Journal | J Biol Chem |
| Volume | 264 |
| Issue | 26 |
| Pagination | 15298-303 |
| Date Published | 1989 Sep 15 |
| ISSN | 0021-9258 |
| Keywords | Alcaligenes, Amino Acid Sequence, Base Sequence, Chromosome Deletion, Cloning, Molecular, Escherichia coli, Gene Amplification, Genes, Genes, Bacterial, Hydroxybutyrates, Molecular Sequence Data, Mutation, Plasmids, Polyesters, Restriction Mapping |
| Abstract | The phbC gene encoding the third enzyme of the poly-beta-hydroxybutyrate biosynthetic pathway, poly-beta-hydroxybutyrate polymerase, in Alcaligenes eutrophus H16 has been identified by the complementation of poly-beta-hydroxybutyrate negative mutants of A. eutrophus H16. These results demonstrate that the three enzymes of the poly-beta-hydroxybutyrate biosynthetic pathway are organized phbC-phbA-phbB. Expression of all three genes in Escherichia coli results in a significant level (50% dry cell weight) of poly-beta-hydroxybutyrate production. phbC encodes a polypeptide of Mr = 63,900 which has a hydropathy profile distinct from typical membrane proteins indicating that poly-beta-hydroxybutyrate biosynthesis probably does not involve a membrane complex. |
| Alternate Journal | J Biol Chem |
| Citation Key | 191 |
| PubMed ID | 2670936 |