Process characteristics of cell lysis mutants of Saccharomyces cerevisiae.

TitleProcess characteristics of cell lysis mutants of Saccharomyces cerevisiae.
Publication TypeJournal Article
Year of Publication1979
AuthorsBoudrant, J, DeAngelo, J, Sinskey, AJ, Tannenbaum, SR
JournalBiotechnol Bioeng
Volume21
Issue4
Pagination659-70
Date Published1979 Apr
ISSN0006-3592
KeywordsAlkaline Phosphatase, Cell Division, Ethanol, Fungal Proteins, Mutation, RNA, Saccharomyces cerevisiae, Temperature
Abstract

Several temperature-sensitive lysis mutants of Saccharomyces cerevisiae were selected according to their ability to release alkaline phosphatase when incubated at a nonpermissive temperature. For two mutants, cell lysis and release of alkaline phosphatase reached a maximum when cells in the logarithmic growth phase were shifted to the nonpermissive temperature. Morphological changes, as well as changes in macromolecular composition of the cells, were observed. Growth is necessary and oxygen is important for the expression of cell lysis at the nonpermissive temperature.

DOI10.1002/bit.260210410
Alternate JournalBiotechnol Bioeng
Citation Key126
PubMed ID369633