Solving Large Problems With Small Biofactories

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2010

MacEachran, Daniel P, Prophete, M E, Sinskey, A J.

The Rhodococcus opacus PD630 heparin-binding hemagglutinin homolog TadA mediates lipid body formation.

Appl Environ Microbiol

76
,

7217-25

(
2010
).

PubMed

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2010

Boccazzi, Paolo, de Almeida, Naomi M, Kurosawa, Kazuhiko, Sinskey, Anthony J.

High-cell-density batch fermentation of Rhodococcus opacus PD630 using a high glucose concentration for triacylglycerol production.

J Biotechnol

147
,

212-8

(
2010
).

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2010

Kurosawa, Kazuhiko, MacEachran, Daniel P, Sinskey, Anthony J.

Antibiotic biosynthesis following horizontal gene transfer: new milestone for novel natural product discovery?

Expert Opin Drug Discov

5
,

819-25

(
2010
).

PubMed

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2008

Barbara, Joanna E, Ghiviriga, Ion, Kurosawa, Kazuhiko, Lessard, Philip A, Rha, ChoKyun, Sambandan, T G, Sinskey, Anthony J.

Rhodostreptomycins, antibiotics biosynthesized following horizontal gene transfer from Streptomyces padanus to Rhodococcus fascians.

J Am Chem Soc

130
,

1126-7

(
2008
).

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2008

Coradetti, Samuel T, Fong, Kit Wah, Goldsipe, Arthur, Rafat, Marjan, Rha, ChoKyun, Sambandan, T G, Sinskey, Anthony J, Stephenson, Brian C.

Association (micellization) and partitioning of aglycon triterpenoids.

J Colloid Interface Sci

325
,

324-30

(
2008
).

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2008

Abdullah, M A, Rahmah, Anisa Ur, Rha, C K, Sinskey, A J.

Cell engineering and molecular pharming for biopharmaceuticals.

Open Med Chem J

2
,

49-61

(
2008
).

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2007

Adenan, I, Govindan, G, Govindan, M, Rha, C K, Sambandan, T G, Sinskey, A, Vanessendelft, J.

A bioactive polyacetylene compound isolated from Centella asiatica.

Planta Med

73
,

597-9

(
2007
).

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2007

Lessard, Philip A, Sinskey, Anthony J, Yang, Joyce C.

Characterization of the mobilization determinants of pAN12, a small replicon from Rhodococcus erythropolis AN12.

Plasmid

57
,

71-81

(
2007
).

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2007

Bramucci, Michael, Lessard, Philip A, Nagarajan, Vasantha, O’Brien, Xian M, Sengupta, Neil, Sinskey, Anthony J, Tomb, Jean-Francois, Windsor, Steven D, Yang, Joyce C.

TraA is required for megaplasmid conjugation in Rhodococcus erythropolis AN12.

Plasmid

57
,

55-70

(
2007
).

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2006

Cardone, M H, Cho, C W, Choi, D S, Kim, C W, Rha, C, Sinskey, A J.

Glioblastoma cell death induced by asiatic acid.

Cell Biol Toxicol

22
,

393-408

(
2006
).

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2006

Bui, V P, Ghiviriga, I, Kurosawa, K, Lessard, P A, Rha, C K, Sambandan, T G, Sinskey, A J, VanEssendelft, J L, Willis, L B.

Characterization of Streptomyces MITKK-103, a newly isolated actinomycin X2-producer.

Appl Microbiol Biotechnol

72
,

145-154

(
2006
).

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2006

Boccazzi, Paolo, Gorret, Nathalie, Jensen, Klavs F, Sinskey, Anthony J, Van Dyk, Tina K, Zanzotto, Andrea.

In situ measurement of bioluminescence and fluorescence in an integrated microbioreactor.

Biotechnol Bioeng

93
,

40-7

(
2006
).

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2006

Bhattacharya, Sanchita, Boccazzi, Paolo, Jensen, Klavs F, Kurosawa, Kazuhiko, Sinskey, Anthony J, Szita, Nicolas, Zhang, Zhiyu.

Differential gene expression profiles and real-time measurements of growth parameters in Saccharomyces cerevisiae grown in microliter-scale bioreactors equipped with internal stirring.

Biotechnol Prog

22
,

710-7

(
2006
).

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2006

Boccazzi, Paolo, Choi, Hyun-Goo, Jensen, Klavs F, Perozziello, Gerardo, Sinskey, Anthony J, Zhang, Zhiyu.

Microchemostat-microbial continuous culture in a polymer-based, instrumented microbioreactor.

Lab Chip

6
,

906-13

(
2006
).

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2006

Archer, John A C, Lessard, Philip, Loos, Andrea, Overhage, Jörg, Plaggenborg, Rainer, Priefert, Horst, Sinskey, Anthony J, Steinbüchel, Alexander.

Potential of Rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol.

Appl Microbiol Biotechnol

72
,

745-55

(
2006
).

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2006

Boccazzi, Paolo, Jensen, Klavs F, Sinskey, Anthony J, Szita, Nicolas, Zhang, Zhiyu.

A well-mixed, polymer-based microbioreactor with integrated optical measurements.

Biotechnol Bioeng

93
,

286-96

(
2006
).

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2006

Crown, William H, Finkelstein, Stan N, Ginsburg, Geoffrey S, Hiller, Arthur J, Huse, Dan, Maguire, Jon, Sinskey, Anthony J, Stallings, Sarah C, Witt, Whitney P.

A framework to evaluate the economic impact of pharmacogenomics.

Pharmacogenomics

7
,

853-62

(
2006
).

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2006

Boccazzi, Paolo, Lee, Harry L T, Ram, Rajeev J, Sinskey, Anthony J.

Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control.

Lab Chip

6
,

1229-35

(
2006
).

PubMed

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2005

Choi, Joonho, Lawrence, Adam G, Rha, ChoKyun, Sinskey, Anthony J, Stubbe, Joanne.

In vitro analysis of the chain termination reaction in the synthesis of poly-(R)-beta-hydroxybutyrate by the class III synthase from Allochromatium vinosum.

Biomacromolecules

6
,

2113-9

(
2005
).

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2005

Sinskey, Anthony J, Stubbe, Joanne, Tian, Jiamin.

Class III polyhydroxybutyrate synthase: involvement in chain termination and reinitiation.

Biochemistry

44
,

8369-77

(
2005
).

PubMed

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2005

Bhattacharya, Sanchita, Boccazzi, Paolo, Jensen, Klavs F, Sinskey, Anthony J, Szita, Nicolas, Zanzotto, Andrea.

Gene expression analysis of Escherichia coli grown in miniaturized bioreactor platforms for high-throughput analysis of growth and genomic data.

Appl Microbiol Biotechnol

68
,

518-32

(
2005
).

PubMed

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2005

Boccazzi, Paolo, Boyle, Patrick, Jensen, Klavs F, Sinskey, Anthony J, Szita, Nicolas, Zhang, Zhiyu.

Development of a multiplexed microbioreactor system for high-throughput bioprocessing.

Lab Chip

5
,

819-26

(
2005
).

PubMed

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2005

He, Aimin, Lawrence, Adam G, Liu, Pinghua, Schoenheit, Joerg, Sinskey, Anthony J, Stubbe, Joanne, Tian, Jiamin.

Transcriptional analysis of Ralstonia eutropha genes related to poly-(R)-3-hydroxybutyrate homeostasis during batch fermentation.

Appl Microbiol Biotechnol

68
,

663-72

(
2005
).

PubMed

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2005

Sinskey, Anthony J, Stubbe, Joanne, Tian, Jiamin.

Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase.

Biochemistry

44
,

1495-503

(
2005
).

PubMed

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2005

He, Aimin, Lawrence, Adam G, Liu, Pinghua, Sinskey, Anthony J, Stubbe, Joanne, Tian, Jiamin.

Nontemplate-dependent polymerization processes: polyhydroxyalkanoate synthases as a paradigm.

Annu Rev Biochem

74
,

433-80

(
2005
).

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2005

Sinskey, Anthony J, Stubbe, Joanne, Tian, Jiamin.

Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy.

J Bacteriol

187
,

3814-24

(
2005
).

PubMed

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2005

He, Aimin, Lawrence, Adam G, Liu, Pinghua, Sinskey, Anthony J, Stubbe, Joanne, Tian, Jiamin, Watson, Nicki.

Analysis of transient polyhydroxybutyrate production in Wautersia eutropha H16 by quantitative Western analysis and transmission electron microscopy.

J Bacteriol

187
,

3825-32

(
2005
).

PubMed

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2004

bin Rosli, Samsul Kamal, Gorret, Nathalie, Lessard, Philip A, Oppenheim, Sheldon F, Rha, ChoKyun, Sinskey, Anthony J, Willis, Laura B.

Bioreactor culture of oil palm (Elaeis guineensis) and effects of nitrogen source, inoculum size, and conditioned medium on biomass production.

J Biotechnol

108
,

253-63

(
2004
).

PubMed

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2004

Currie, Devin H, Lessard, Philip A, O’Brien, Xian M, Sinskey, Anthony J.

pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus.

BMC Microbiol

4
,

15

(
2004
).

PubMed

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2004

Boccazzi, Paolo, Jensen, Klavs F, Lessard, Philip, Sinskey, Anthony J, Szita, Nicolas, Zanzotto, Andrea.

Membrane-aerated microbioreactor for high-throughput bioprocessing.

Biotechnol Bioeng

87
,

243-54

(
2004
).

PubMed

DOI

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2004

Agosto, Melina, Cho, Jennie J, Choi, Ellen E, Dexter, Annette F, Lessard, Philip A, Nagpal, Geeta, O’Brien, Xian M, Priefert, Horst, Sinskey, Anthony J, Tamashiro, Lance, Tomic, Sladjana, Treadway, Sheri L, Wallace, Matthew, Yang, Lucy.

Indene bioconversion by a toluene inducible dioxygenase of Rhodococcus sp. I24.

Appl Microbiol Biotechnol

65
,

168-76

(
2004
).

PubMed

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2004

Finkelstein, Stan N, Hawkins, Kevin, Marder, William D, Ozminkowski, Ronald J, Sinskey, Anthony J, Stallings, Sarah C, Wang, Shaohung, Wierz, David.

The use of disease-modifying new drugs for multiple sclerosis treatment in private-sector health plans.

Clin Ther

26
,

1341-54

(
2004
).

PubMed

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2003

Ali, Johari Mohd, Coldren, Christopher D, Hashim, Puziah, Oh, Se-Kyung, Rha, ChoKyun, Sinskey, Anthony J.

Gene expression changes in the human fibroblast induced by Centella asiatica triterpenoids.

Planta Med

69
,

725-32

(
2003
).

PubMed

DOI

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2003

Haron, M Rosli, Housman, David E, Jordan, Barbara, Lessard, Philip A, Muhammad, Norwati, Osman, Asiah, Rha, ChoKyun, Riffin, Norifiza Mat, Sinskey, Anthony J.

Genetic diversity of Eurycoma longifolia inferred from single nucleotide polymorphisms.

Plant Physiol

131
,

1294-301

(
2003
).

PubMed

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2003

Glanemann, C, Gorret, N, Lessard, P A, Loos, A, O’Brien, X M, Sinskey, A J, Willis, L B.

Disparity between changes in mRNA abundance and enzyme activity in Corynebacterium glutamicum: implications for DNA microarray analysis.

Appl Microbiol Biotechnol

61
,

61-8

(
2003
).

PubMed

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2003

Cardone, Mike H, MacBeath, Gavin, Nielsen, Ulrik B, Sinskey, Anthony J, Sorger, Peter K.

Profiling receptor tyrosine kinase activation by using Ab microarrays.

Proc Natl Acad Sci U S A

100
,

9330-5

(
2003
).

PubMed

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2003

Lawrence, Adam G, Lupberger, Joachim, Sinskey, Anthony J, Stubbe, Joanne, Tian, Jiamin, York, Gregory M.

Ralstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genes.

J Bacteriol

185
,

3788-94

(
2003
).

PubMed

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2002

Kulaveerasingam, Harikrishna, Lessard, Philip A, Sinskey, Anthony J, Strong, Amie, York, Gregory M.

Manipulating gene expression for the metabolic engineering of plants.

Metab Eng

4
,

67-79

(
2002
).

PubMed

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2002

Sinskey, Anthony J, Stubbe, Joanne, York, Gregory M.

The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production.

J Bacteriol

184
,

59-66

(
2002
).

PubMed

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2002

Lessard, Philip A, Rijhwani, Sushil K, Sinskey, Anthony J, Stafford, Daniel E, Stephanopoulos, Gregory, Yanagimachi, Kurt S.

Optimizing bioconversion pathways through systems analysis and metabolic engineering.

Proc Natl Acad Sci U S A

99
,

1801-6

(
2002
).

PubMed

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2002

Lessard, P A, O’Brien, X M, Parker, J A, Sinskey, A J.

Engineering an indene bioconversion process for the production of cis-aminoindanol: a model system for the production of chiral synthons.

Appl Microbiol Biotechnol

59
,

389-99

(
2002
).

PubMed

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2001

Gerstmeir, R, Glanemann, C, Guillouet, S, Lessard, P A, Loos, A, O’Brien, X M, Sinskey, A J, Willis, L B.

Development and validation of corynebacterium DNA microarrays.

Appl Environ Microbiol

67
,

2310-8

(
2001
).

PubMed

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2001

Dexter, A F, Drew, S, Sinskey, A J, Stafford, D E, Stephanopoulos, G, Yanagimachi, K S.

Application of radiolabeled tracers to biocatalytic flux analysis.

Eur J Biochem

268
,

4950-60

(
2001
).

PubMed

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2001

An, G H, Gorret, N, Guillouet, S, Lessard, P A, Rodal, A A, Sinskey, A J.

Metabolic redirection of carbon flow toward isoleucine by expressing a catabolic threonine dehydratase in a threonine-overproducing Corynebacterium glutamicum.

Appl Microbiol Biotechnol

57
,

667-73

(
2001
).

PubMed

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2001

Sinskey, A J, Stubbe, J, York, G M.

New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate.

J Bacteriol

183
,

2394-7

(
2001
).

PubMed

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2001

Junker, B H, Sinskey, A J, Stubbe, J A, York, G M.

Accumulation of the PhaP phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells.

J Bacteriol

183
,

4217-26

(
2001
).

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2001

Jia, Y, Park, C, Sinskey, A J, Stubbe, J, Wodzinska, J, Yuan, W.

Mechanistic studies on class I polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha: class I and III synthases share a similar catalytic mechanism.

Biochemistry

40
,

1011-9

(
2001
).

PubMed

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2001

Jia, Y, Lambalot, R H, Müh, U, Sinskey, A J, Snell, K D, Stubbe, J, Tian, J, Walsh, C T, Yuan, W.

Class I and III polyhydroxyalkanoate synthases from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies.

Arch Biochem Biophys

394
,

87-98

(
2001
).

PubMed

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2000

Frick, T, Jia, Y, Kappock, T J, Sinskey, A J, Stubbe, J.

Lipases provide a new mechanistic model for polyhydroxybutyrate (PHB) synthases: characterization of the functional residues in Chromatium vinosum PHB synthase.

Biochemistry

39
,

3927-36

(
2000
).

PubMed

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1999

Klapa, M I, Park, S M, Sinskey, A J, Stephanopoulos, G.

Metabolite and isotopomer balancing in the analysis of metabolic cycles: II. Applications.

Biotechnol Bioeng

62
,

392-401

(
1999
).

PubMed

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