Solving Large Problems With Small Biofactories

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1999

Klapa, MI, Park, SM, Sinskey, AJ, Stephanopoulos, G.

Metabolite and isotopomer balancing in the analysis of metabolic cycles: I. Theory.

Biotechnol Bioeng

62
,

375-391

(
1999
).

PubMed

DOI

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1999

An, G, Guillouet, S, Lessard, P A, Rodal, A A, Sinskey, A J.

Expression of the Escherichia coli catabolic threonine dehydratase in Corynebacterium glutamicum and its effect on isoleucine production.

Appl Environ Microbiol

65
,

3100-7

(
1999
).

PubMed

DOI

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1999

An, G H, Sinskey, A J, Song, K B.

Redirection of carbon flux to lysine in a recombinant of Corynebacterium lactofermentum ATCC 21799 by limited supply of pantothenate.

J Biosci Bioeng

88
,

168-72

(
1999
).

PubMed

DOI

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1999

Kirby, D P, Lane, W S, Müh, U, Sinskey, A J, Stubbe, J.

PHA synthase from chromatium vinosum: cysteine 149 is involved in covalent catalysis.

Biochemistry

38
,

826-37

(
1999
).

PubMed

DOI

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1999

Goswami, J, Sinskey, A J, Steller, H, Stephanopoulos, G N, Wang, D I.

Apoptosis in batch cultures of Chinese hamster ovary cells.

Biotechnol Bioeng

62
,

632-40

(
1999
).

PubMed

DOI

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1999

Ahlgren, N A, Lessard, P A, O’Brien, X M, Ribich, S A, Sinskey, A J.

Characterization of IS1676 from Rhodococcus erythropolis SQ1.

Appl Microbiol Biotechnol

52
,

811-9

(
1999
).

PubMed

DOI

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1999

Lankenau, E, Lessard, P A, Sinskey, A J, Stephanopoulos, G, Treadway, S L, Yanagimachi, K S.

Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24.

Appl Microbiol Biotechnol

51
,

786-93

(
1999
).

PubMed

DOI

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1999

McCormick, M M, Shaw-Reid, C A, Sinskey, A J, Stephanopoulos, G.

Flux through the tetrahydrodipicolinate succinylase pathway is dispensable for L-lysine production in Corynebacterium glutamicum.

Appl Microbiol Biotechnol

51
,

325-33

(
1999
).

PubMed

DOI

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1998

Chung, J D, Sinskey, A J, Stephanopoulos, G.

Growth factor and bcl-2 mediated survival during abortive proliferation of hybridoma cell line.

Biotechnol Bioeng

57
,

164-71

(
1998
).

PubMed

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1998

Koffas, M A, Pine, W A, Ramamoorthi, R, Sinskey, A J, Stephanopoulos, G.

Sequence of the Corynebacterium glutamicum pyruvate carboxylase gene.

Appl Microbiol Biotechnol

50
,

346-52

(
1998
).

PubMed

DOI

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1997

Hogan, S A, Rha, C, Sim, S J, Sinskey, A J, Snell, K D, Stubbe, J.

PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo.

Nat Biotechnol

15
,

63-7

(
1997
).

PubMed

DOI

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1997

Park, S M, Sinskey, A J, Stephanopoulos, G.

Metabolic and physiological studies of Corynebacterium glutamicum mutants.

Biotechnol Bioeng

55
,

864-79

(
1997
).

PubMed

DOI

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1997

Chung, J D, Sinskey, A J, Stephanopoulos, G, Zabel, C.

Extension of Sp2/0 hybridoma cell viability through interleukin-6 supplementation.

Biotechnol Bioeng

55
,

439-46

(
1997
).

PubMed

DOI

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1996

Shelikoff, M, Sinskey, A J, Stephanopoulos, G.

A modeling framework for the study of protein glycosylation.

Biotechnol Bioeng

50
,

73-90

(
1996
).

PubMed

DOI

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1995

Hwang, C, Lodish, H F, Sinskey, A J.

Measurement of glutathione redox state in cytosol and secretory pathway of cultured cells.

Methods Enzymol

251
,

212-21

(
1995
).

PubMed

DOI

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1995

Colón, G E, Follettie, M T, Gubler, M E, Jetten, M S, Nguyen, T T, Sinskey, A J, Stephanopoulos, G.

Effect of inducible thrB expression on amino acid production in Corynebacterium lactofermentum ATCC 21799.

Appl Environ Microbiol

61
,

74-8

(
1995
).

PubMed

DOI

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1995

Colón, G E, Jetten, M S, Nguyen, T T, Sinskey, A J, Stephanopoulos, G.

Production of isoleucine by overexpression of ilvA in a Corynebacterium lactofermentum threonine producer.

Appl Microbiol Biotechnol

43
,

482-8

(
1995
).

PubMed

DOI

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1995

Jetten, M S, Sinskey, A J.

Purification and properties of oxaloacetate decarboxylase from Corynebacterium glutamicum.

Antonie Van Leeuwenhoek

67
,

221-7

(
1995
).

PubMed

DOI

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1995

Follettie, M T, Jetten, M S, Sinskey, A J.

Effect of different levels of aspartokinase on the lysine production by Corynebacterium lactofermentum.

Appl Microbiol Biotechnol

43
,

76-82

(
1995
).

PubMed

DOI

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1995

Chen, K, Lodish, H F, Sinskey, A J, Stephanopoulos, G N.

Regulated secretion of prolactin by the mouse insulinoma cell line beta TC-3.

Biotechnology (N Y)

13
,

1191-7

(
1995
).

PubMed

DOI

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1995

Sinskey, A J, Stephanopoulos, G, Zupke, C.

Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas.

Appl Microbiol Biotechnol

44
,

27-36

(
1995
).

PubMed

DOI

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1995

Jetten, M S, Sinskey, A J.

Recent advances in the physiology and genetics of amino acid-producing bacteria.

Crit Rev Biotechnol

15
,

73-103

(
1995
).

PubMed

DOI

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1994

Shelikoff, M, Sinskey, A J, Stephanopoulos, G.

The effect of protein synthesis inhibitors on the glycosylation site occupancy of recombinant human prolactin.

Cytotechnology

15
,

195-208

(
1994
).

PubMed

DOI

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1994

Gubler, M E, Jetten, M S, Lee, S H, Sinskey, A J.

Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum.

Appl Environ Microbiol

60
,

2501-7

(
1994
).

PubMed

DOI

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1994

Follettie, M T, Jetten, M S, Sinskey, A J.

Metabolic engineering of Corynebacterium glutamicum.

Ann N Y Acad Sci

721
,

12-29

(
1994
).

PubMed

DOI

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1994

Csuhai, E, Gerngross, T U, Masamune, S, Peoples, O P, Sinskey, A J, Snell, K D, Stubbe, J.

Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity.

Biochemistry

33
,

9311-20

(
1994
).

PubMed

DOI

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1994

Gubler, M, Jetten, M, Lee, S H, Sinskey, A J.

Cloning of the pyruvate kinase gene (pyk) of Corynebacterium glutamicum and site-specific inactivation of pyk in a lysine-producing Corynebacterium lactofermentum strain.

Appl Environ Microbiol

60
,

2494-500

(
1994
).

PubMed

DOI

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1993

Sinskey, A J, Stephanopoulos, G.

Metabolic engineering–methodologies and future prospects.

Trends Biotechnol

11
,

392-6

(
1993
).

PubMed

DOI

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1993

Archer, J A, Sinskey, A J.

The DNA sequence and minimal replicon of the Corynebacterium glutamicum plasmid pSR1: evidence of a common ancestry with plasmids from C. diphtheriae.

J Gen Microbiol

139
,

1753-9

(
1993
).

PubMed

DOI

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1993

Gerngross, T U, Peoples, O P, Reilly, P, Sinskey, A J, Stubbe, J.

Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules.

J Bacteriol

175
,

5289-93

(
1993
).

PubMed

DOI

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1993

Agoropoulou, C, Follettie, M T, Peoples, O P, Sinskey, A J.

Gene structure and expression of the Corynebacterium flavum N13 ask-asd operon.

J Bacteriol

175
,

4096-103

(
1993
).

PubMed

DOI

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1992

Hwang, C, Lodish, H F, Sinskey, A J.

Oxidized redox state of glutathione in the endoplasmic reticulum.

Science

257
,

1496-502

(
1992
).

PubMed

DOI

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1992

Masamune, S, Palmer, M A, Peoples, O P, Sinskey, A J, Walsh, C T, Williams, S F.

Biosynthetic thiolase from Zoogloea ramigera. Mutagenesis of the putative active-site base Cys-378 to Ser-378 changes the partitioning of the acetyl S-enzyme intermediate.

J Biol Chem

267
,

16041-3

(
1992
).

PubMed

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1992

Liebl, W, Schleifer, K H, Sinskey, A J.

Expression, secretion, and processing of staphylococcal nuclease by Corynebacterium glutamicum.

J Bacteriol

174
,

1854-61

(
1992
).

PubMed

DOI

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1992

Jain, J, Rao, A, Sinskey, A J, Valge-Archer, V E.

The AP-1 site at -150 bp, but not the NF-kappa B site, is likely to represent the major target of protein kinase C in the interleukin 2 promoter.

J Exp Med

175
,

853-62

(
1992
).

PubMed

DOI

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1992

Batt, C A, Bor, Y C, Crosby, W L, Lee, S P, Moraes, C, Sinskey, A J.

Cloning and sequencing the Lactobacillus brevis gene encoding xylose isomerase.

Gene

114
,

127-32

(
1992
).

PubMed

DOI

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1991

Archer, J A, Sinskey, A J, Solow-Cordero, D E.

A C-terminal deletion in Corynebacterium glutamicum homoserine dehydrogenase abolishes allosteric inhibition by L-threonine.

Gene

107
,

53-9

(
1991
).

PubMed

DOI

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1991

Differding, E, Gamboni, R, Masamune, S, Palmer, M A, Peoples, O P, Sinskey, A J, Walsh, C T, Williams, S F.

Biosynthetic thiolase from Zoogloea ramigera. Evidence for a mechanism involving Cys-378 as the active site base.

J Biol Chem

266
,

8369-75

(
1991
).

PubMed

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1990

Lodish, H F, Sambanis, A, Sinskey, A J, Stephanopoulos, G.

Use of regulated secretion in protein production from animal cells: an evaluation with the AtT-20 model cell line.

Biotechnol Bioeng

35
,

771-80

(
1990
).

PubMed

DOI

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1990

Archer, J A, Marcel, T, Mengin-Lecreulx, D, Sinskey, A J.

Nucleotide sequence and organization of the upstream region of the Corynebacterium glutamicum lysA gene.

Mol Microbiol

4
,

1819-30

(
1990
).

PubMed

DOI

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1990

de Villiers, J, Rao, A, Sinskey, A J, Valge-Archer, V E.

Transformation of T lymphocytes by the v-fos oncogene.

J Immunol

145
,

4355-64

(
1990
).

PubMed

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1990

Archer, J A, Han, K S, Sinskey, A J.

The molecular structure of the Corynebacterium glutamicum threonine synthase gene.

Mol Microbiol

4
,

1693-702

(
1990
).

PubMed

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1990

Barbas, C, Gioannetti, C, Ozaki, A, Pederson, R L, Sinskey, A J, Toone, E, von der Osten, C H, Wang, Y F, Whitesides, G M, Wong, C H.

Molecular cloning of aldolases for synthetic applications.

Ann N Y Acad Sci

613
,

771-5

(
1990
).

PubMed

DOI

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1989

Masamune, S, Mayerl, F, Peoples, O P, Sinskey, A J, Thompson, S, Walsh, C T.

Mechanistic studies on beta-ketoacyl thiolase from Zoogloea ramigera: identification of the active-site nucleophile as Cys89, its mutation to Ser89, and kinetic and thermodynamic characterization of wild-type and mutant enzymes.

Biochemistry

28
,

5735-42

(
1989
).

PubMed

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1989

Barbas, C F, Sinskey, A J, von der Osten, C H, Wong, C H.

Molecular cloning, nucleotide sequence and fine-structural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-1,6-biphosphate aldolase to class I and class II aldolases.

Mol Microbiol

3
,

1625-37

(
1989
).

PubMed

DOI

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1989

Peoples, O P, Sinskey, A J.

Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC).

J Biol Chem

264
,

15298-303

(
1989
).

PubMed

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1989

Peoples, O P, Sinskey, A J.

Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase.

J Biol Chem

264
,

15293-7

(
1989
).

PubMed

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1989

Eikmanns, B J, Follettie, M T, Griot, M U, Sinskey, A J.

The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucleotide sequence, and expression.

Mol Gen Genet

218
,

330-9

(
1989
).

PubMed

DOI

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1989

Peoples, O P, Sinskey, A J.

Fine structural analysis of the Zoogloea ramigera phbA-phbB locus encoding beta-ketothiolase and acetoacetyl-CoA reductase: nucleotide sequence of phbB.

Mol Microbiol

3
,

349-57

(
1989
).

PubMed

DOI

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1989

Adema, E, Glacken, M W, Sinskey, A J.

Mathematical descriptions of hybridoma culture kinetics: II. The relationship between thiol chemistry and the degradation of serum activity.

Biotechnol Bioeng

33
,

440-50

(
1989
).

PubMed

DOI

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