Publications

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Journal Article
Cao, J. et al. Yeast-Based Synthetic Biology Platform for Antimicrobial Peptide Production. ACS Synth Biol 7, 896-902 (2018).
Brigham, C. J., Speth, D. R., Rha, C. K. & Sinskey, A. J. Whole-genome microarray and gene deletion studies reveal regulation of the polyhydroxyalkanoate production cycle by the stringent response in Ralstonia eutropha H16. Appl Environ Microbiol 78, 8033-44 (2012).
Zhang, Z., Szita, N., Boccazzi, P., Sinskey, A. J. & Jensen, K. F. A well-mixed, polymer-based microbioreactor with integrated optical measurements. Biotechnol Bioeng 93, 286-96 (2006).
Ozminkowski, R. J. et al. The use of disease-modifying new drugs for multiple sclerosis treatment in private-sector health plans. Clin Ther 26, 1341-54 (2004).
Puglisi, E. et al. Transcriptional response of Rhodococcus aetherivorans I24 to polychlorinated biphenyl-contaminated sediments. Microb Ecol 60, 505-15 (2010).
Lawrence, A. G. et al. Transcriptional analysis of Ralstonia eutropha genes related to poly-(R)-3-hydroxybutyrate homeostasis during batch fermentation. Appl Microbiol Biotechnol 68, 663-72 (2005).
Yang, J. C. et al. TraA is required for megaplasmid conjugation in Rhodococcus erythropolis AN12. Plasmid 57, 55-70 (2007).
Kurosawa, K., Laser, J. & Sinskey, A. J. Tolerance and adaptive evolution of triacylglycerol-producing Rhodococcus opacus to lignocellulose-derived inhibitors. Biotechnol Biofuels 8, 76 (2015).
Lu, J., Brigham, C. J., Gai, C. S. & Sinskey, A. J. Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha. Appl Microbiol Biotechnol 96, 283-97 (2012).
Budde, C. F., Mahan, A. E., Lu, J., Rha, C. K. & Sinskey, A. J. Roles of multiple acetoacetyl coenzyme A reductases in polyhydroxybutyrate biosynthesis in Ralstonia eutropha H16. J Bacteriol 192, 5319-28 (2010).
Kurosawa, K. et al. Rhodostreptomycins, antibiotics biosynthesized following horizontal gene transfer from Streptomyces padanus to Rhodococcus fascians. J Am Chem Soc 130, 1126-7 (2008).
MacEachran, D. P. & Sinskey, A. J. The Rhodococcus opacus TadD protein mediates triacylglycerol metabolism by regulating intracellular NAD(P)H pools. Microb Cell Fact 12, 104 (2013).
Riedel, S. L. et al. Recovery of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Ralstonia eutropha cultures with non-halogenated solvents. Biotechnol Bioeng 110, 461-70 (2013).
York, G. M., Stubbe, J. & Sinskey, A. J. The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production. J Bacteriol 184, 59-66 (2002).
York, G. M. et al. Ralstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genes. J Bacteriol 185, 3788-94 (2003).
Cho, M., Brigham, C. J., Sinskey, A. J. & Stubbe, J. Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo. Biochemistry 51, 2276-88 (2012).
Nielsen, U. B., Cardone, M. H., Sinskey, A. J., MacBeath, G. & Sorger, P. K. Profiling receptor tyrosine kinase activation by using Ab microarrays. Proc Natl Acad Sci U S A 100, 9330-5 (2003).
Budde, C. F., Riedel, S. L., Willis, L. B., Rha, C. K. & Sinskey, A. J. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl Environ Microbiol 77, 2847-54 (2011).
Riedel, S. L. et al. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Ralstonia eutropha in high cell density palm oil fermentations. Biotechnol Bioeng 109, 74-83 (2012).
Purcell, O. et al. Production of Functional Anti-Ebola Antibodies in Pichia pastoris. ACS Synth Biol 6, 2183-2190 (2017).
Plaggenborg, R. et al. Potential of Rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol. Appl Microbiol Biotechnol 72, 745-55 (2006).
Sambanthamurthi, R. et al. Positive outcomes of oil palm phenolics on degenerative diseases in animal models. Br J Nutr 106, 1664-75 (2011).
Lessard, P. A., O'Brien, X. M., Currie, D. H. & Sinskey, A. J. pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus. BMC Microbiol 4, 15 (2004).
Marc, J. et al. Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production. Metab Eng 42, 74-84 (2017).
Stafford, D. E. et al. Optimizing bioconversion pathways through systems analysis and metabolic engineering. Proc Natl Acad Sci U S A 99, 1801-6 (2002).
Yang, Y. - H. et al. Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. Appl Microbiol Biotechnol 87, 2037-45 (2010).
Sambanthamurthi, R. et al. Oil palm vegetation liquor: a new source of phenolic bioactives. Br J Nutr 106, 1655-63 (2011).
Weinberg, R. P. et al. Oil Palm Phenolics Inhibit the Aggregation of -Amyloid Peptide into Oligomeric Complexes. Int J Alzheimers Dis 2018, 7608038 (2018).
Stubbe, J. et al. Nontemplate-dependent polymerization processes: polyhydroxyalkanoate synthases as a paradigm. Annu Rev Biochem 74, 433-80 (2005).
Fuchs, B. C. et al. Molecular MRI of collagen to diagnose and stage liver fibrosis. J Hepatol 59, 992-8 (2013).
Lee, K. S., Boccazzi, P., Sinskey, A. J. & Ram, R. J. Microfluidic chemostat and turbidostat with flow rate, oxygen, and temperature control for dynamic continuous culture. Lab Chip 11, 1730-9 (2011).
Zhang, Z. et al. Microchemostat-microbial continuous culture in a polymer-based, instrumented microbioreactor. Lab Chip 6, 906-13 (2006).
Lee, H. L. T., Boccazzi, P., Ram, R. J. & Sinskey, A. J. Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control. Lab Chip 6, 1229-35 (2006).
Plassmeier, J., Li, Y., Rueckert, C. & Sinskey, A. J. Metabolic engineering Corynebacterium glutamicum to produce triacylglycerols. Metab Eng 33, 86-97 (2016).
Zanzotto, A. et al. Membrane-aerated microbioreactor for high-throughput bioprocessing. Biotechnol Bioeng 87, 243-54 (2004).
Brigham, C. J., Zhila, N., Shishatskaya, E., Volova, T. G. & Sinskey, A. J. Manipulation of Ralstonia eutropha carbon storage pathways to produce useful bio-based products. Subcell Biochem 64, 343-66 (2012).
Lessard, P. A., Kulaveerasingam, H., York, G. M., Strong, A. & Sinskey, A. J. Manipulating gene expression for the metabolic engineering of plants. Metab Eng 4, 67-79 (2002).
Kurosawa, K., C Debono, A. & Sinskey, A. J. Lignocellulose-derived inhibitors improve lipid extraction from wet Rhodococcus opacus cells. Bioresour Technol 193, 206-12 (2015).
Tian, J., Sinskey, A. J. & Stubbe, J. Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy. J Bacteriol 187, 3814-24 (2005).
Grousseau, E., Lu, J., Gorret, N., Guillouet, S. E. & Sinskey, A. J. Isopropanol production with engineered Cupriavidus necator as bioproduction platform. Appl Microbiol Biotechnol 98, 4277-90 (2014).
Gai, C. S., Lu, J., Brigham, C. J., Bernardi, A. C. & Sinskey, A. J. Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16. AMB Express 4, 2 (2014).
Priefert, H. et al. Indene bioconversion by a toluene inducible dioxygenase of Rhodococcus sp. I24. Appl Microbiol Biotechnol 65, 168-76 (2004).
Lawrence, A. G., Choi, J., Rha, C. K., Stubbe, J. & Sinskey, A. J. In vitro analysis of the chain termination reaction in the synthesis of poly-(R)-beta-hydroxybutyrate by the class III synthase from Allochromatium vinosum. Biomacromolecules 6, 2113-9 (2005).
Zanzotto, A. et al. In situ measurement of bioluminescence and fluorescence in an integrated microbioreactor. Biotechnol Bioeng 93, 40-7 (2006).
Kurosawa, K., Radek, A., Plassmeier, J. K. & Sinskey, A. J. Improved glycerol utilization by a triacylglycerol-producing Rhodococcus opacus strain for renewable fuels. Biotechnol Biofuels 8, 31 (2015).
Kurosawa, K., Boccazzi, P., de Almeida, N. M. & Sinskey, A. J. High-cell-density batch fermentation of Rhodococcus opacus PD630 using a high glucose concentration for triacylglycerol production. J Biotechnol 147, 212-8 (2010).
Budde, C. F. et al. Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium. Appl Microbiol Biotechnol 89, 1611-9 (2011).
Osman, A. et al. Genetic diversity of Eurycoma longifolia inferred from single nucleotide polymorphisms. Plant Physiol 131, 1294-301 (2003).
Coldren, C. D. et al. Gene expression changes in the human fibroblast induced by Centella asiatica triterpenoids. Planta Med 69, 725-32 (2003).
Boccazzi, P. et al. Gene expression analysis of Escherichia coli grown in miniaturized bioreactor platforms for high-throughput analysis of growth and genomic data. Appl Microbiol Biotechnol 68, 518-32 (2005).

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