Publications

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2018
Weinberg, R. P. et al. Oil Palm Phenolics Inhibit the Aggregation of -Amyloid Peptide into Oligomeric Complexes. Int J Alzheimers Dis 2018, 7608038 (2018).
Cao, J. et al. Yeast-Based Synthetic Biology Platform for Antimicrobial Peptide Production. ACS Synth Biol 7, 896-902 (2018).
2017
Juengert, J. R. et al. Absence of ppGpp Leads to Increased Mobilization of Intermediately Accumulated Poly(3-Hydroxybutyrate) in Ralstonia eutropha H16. Appl Environ Microbiol 83, (2017).
Brigham, C. J., Speth, D. R., Rha, C. K. & Sinskey, A. J. Correction for Brigham et al., "Whole-Genome Microarray and Gene Deletion Studies Reveal Regulation of the Polyhydroxyalkanoate Production Cycle by the Stringent Response in Ralstonia eutropha H16". Appl Environ Microbiol 83, (2017).
Bernardi, A. C., Gai, C. S., Lu, J., Sinskey, A. J. & Brigham, C. J. Corrigendum to "Experimental evolution and gene knockout studies reveal AcrA-mediated isobutanol tolerance in Ralstonia eutropha" [J Biosci Bioeng 122 (2016) 64-69]. J Biosci Bioeng 123, 658 (2017).
Marc, J. et al. Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production. Metab Eng 42, 74-84 (2017).
Purcell, O. et al. Production of Functional Anti-Ebola Antibodies in Pichia pastoris. ACS Synth Biol 6, 2183-2190 (2017).
2016
Bernardi, A. C., Gai, C. S., Lu, J., Sinskey, A. J. & Brigham, C. J. Experimental evolution and gene knockout studies reveal AcrA-mediated isobutanol tolerance in Ralstonia eutropha. J Biosci Bioeng 122, 64-9 (2016).
Plassmeier, J., Li, Y., Rueckert, C. & Sinskey, A. J. Metabolic engineering Corynebacterium glutamicum to produce triacylglycerols. Metab Eng 33, 86-97 (2016).
2015
Farrar, C. T. et al. 3D molecular MR imaging of liver fibrosis and response to rapamycin therapy in a bile duct ligation rat model. J Hepatol 63, 689-96 (2015).
Lu, J., Brigham, C. J., Plassmeier, J. K. & Sinskey, A. J. Characterization and modification of enzymes in the 2-ketoisovalerate biosynthesis pathway of Ralstonia eutropha H16. Appl Microbiol Biotechnol 99, 761-74 (2015).
Kurosawa, K., Plassmeier, J., Kalinowski, J., Rückert, C. & Sinskey, A. J. Engineering L-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Metab Eng 30, 89-95 (2015).
Kurosawa, K., Radek, A., Plassmeier, J. K. & Sinskey, A. J. Improved glycerol utilization by a triacylglycerol-producing Rhodococcus opacus strain for renewable fuels. Biotechnol Biofuels 8, 31 (2015).
Kurosawa, K., C Debono, A. & Sinskey, A. J. Lignocellulose-derived inhibitors improve lipid extraction from wet Rhodococcus opacus cells. Bioresour Technol 193, 206-12 (2015).
Kurosawa, K., Laser, J. & Sinskey, A. J. Tolerance and adaptive evolution of triacylglycerol-producing Rhodococcus opacus to lignocellulose-derived inhibitors. Biotechnol Biofuels 8, 76 (2015).
2014
Jeon, J. - M. et al. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha. Appl Microbiol Biotechnol 98, 5461-9 (2014).
Gai, C. S., Lu, J., Brigham, C. J., Bernardi, A. C. & Sinskey, A. J. Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16. AMB Express 4, 2 (2014).
Grousseau, E., Lu, J., Gorret, N., Guillouet, S. E. & Sinskey, A. J. Isopropanol production with engineered Cupriavidus necator as bioproduction platform. Appl Microbiol Biotechnol 98, 4277-90 (2014).
2013
Lu, J., Brigham, C. J., Rha, C. K. & Sinskey, A. J. Characterization of an extracellular lipase and its chaperone from Ralstonia eutropha H16. Appl Microbiol Biotechnol 97, 2443-54 (2013).
Volova, T. G., Zhila, N. O., Kalacheva, G. S., Brigham, C. J. & Sinskey, A. J. Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha. Res Microbiol 164, 164-71 (2013).
Kurosawa, K., Wewetzer, S. J. & Sinskey, A. J. Engineering xylose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Biotechnol Biofuels 6, 134 (2013).
Fuchs, B. C. et al. Molecular MRI of collagen to diagnose and stage liver fibrosis. J Hepatol 59, 992-8 (2013).
Riedel, S. L. et al. Recovery of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Ralstonia eutropha cultures with non-halogenated solvents. Biotechnol Bioeng 110, 461-70 (2013).
MacEachran, D. P. & Sinskey, A. J. The Rhodococcus opacus TadD protein mediates triacylglycerol metabolism by regulating intracellular NAD(P)H pools. Microb Cell Fact 12, 104 (2013).
2012
Wong, Y. - M., Brigham, C. J., Rha, C. K., Sinskey, A. J. & Sudesh, K. Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator. Bioresour Technol 121, 320-7 (2012).
Song, Y. et al. Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production. Appl Microbiol Biotechnol 93, 1917-25 (2012).
Brigham, C. J., Reimer, E. N., Rha, C. K. & Sinskey, A. J. Examination of PHB Depolymerases in Ralstonia eutropha: Further Elucidation of the Roles of Enzymes in PHB Homeostasis. AMB Express 2, 26 (2012).
Brigham, C. J., Zhila, N., Shishatskaya, E., Volova, T. G. & Sinskey, A. J. Manipulation of Ralstonia eutropha carbon storage pathways to produce useful bio-based products. Subcell Biochem 64, 343-66 (2012).
Riedel, S. L. et al. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Ralstonia eutropha in high cell density palm oil fermentations. Biotechnol Bioeng 109, 74-83 (2012).
Cho, M., Brigham, C. J., Sinskey, A. J. & Stubbe, J. Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo. Biochemistry 51, 2276-88 (2012).
Lu, J., Brigham, C. J., Gai, C. S. & Sinskey, A. J. Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha. Appl Microbiol Biotechnol 96, 283-97 (2012).
Brigham, C. J., Speth, D. R., Rha, C. K. & Sinskey, A. J. Whole-genome microarray and gene deletion studies reveal regulation of the polyhydroxyalkanoate production cycle by the stringent response in Ralstonia eutropha H16. Appl Environ Microbiol 78, 8033-44 (2012).
2011
Bhubalan, K. et al. Characterization of the highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2. Appl Environ Microbiol 77, 2926-33 (2011).
Holder, J. W. et al. Comparative and functional genomics of Rhodococcus opacus PD630 for biofuels development. PLoS Genet 7, e1002219 (2011).
Budde, C. F. et al. Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium. Appl Microbiol Biotechnol 89, 1611-9 (2011).
Lee, K. S., Boccazzi, P., Sinskey, A. J. & Ram, R. J. Microfluidic chemostat and turbidostat with flow rate, oxygen, and temperature control for dynamic continuous culture. Lab Chip 11, 1730-9 (2011).
Sambanthamurthi, R. et al. Oil palm vegetation liquor: a new source of phenolic bioactives. Br J Nutr 106, 1655-63 (2011).
Sambanthamurthi, R. et al. Positive outcomes of oil palm phenolics on degenerative diseases in animal models. Br J Nutr 106, 1664-75 (2011).
Budde, C. F., Riedel, S. L., Willis, L. B., Rha, C. K. & Sinskey, A. J. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl Environ Microbiol 77, 2847-54 (2011).
2010
Kurosawa, K., MacEachran, D. P. & Sinskey, A. J. Antibiotic biosynthesis following horizontal gene transfer: new milestone for novel natural product discovery?. Expert Opin Drug Discov 5, 819-25 (2010).
Brigham, C. J. et al. Elucidation of beta-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression. J Bacteriol 192, 5454-64 (2010).
Kurosawa, K., Boccazzi, P., de Almeida, N. M. & Sinskey, A. J. High-cell-density batch fermentation of Rhodococcus opacus PD630 using a high glucose concentration for triacylglycerol production. J Biotechnol 147, 212-8 (2010).
Yang, Y. - H. et al. Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. Appl Microbiol Biotechnol 87, 2037-45 (2010).
Budde, C. F., Mahan, A. E., Lu, J., Rha, C. K. & Sinskey, A. J. Roles of multiple acetoacetyl coenzyme A reductases in polyhydroxybutyrate biosynthesis in Ralstonia eutropha H16. J Bacteriol 192, 5319-28 (2010).
Puglisi, E. et al. Transcriptional response of Rhodococcus aetherivorans I24 to polychlorinated biphenyl-contaminated sediments. Microb Ecol 60, 505-15 (2010).
2008
Rafat, M. et al. Association (micellization) and partitioning of aglycon triterpenoids. J Colloid Interface Sci 325, 324-30 (2008).
Kurosawa, K. et al. Rhodostreptomycins, antibiotics biosynthesized following horizontal gene transfer from Streptomyces padanus to Rhodococcus fascians. J Am Chem Soc 130, 1126-7 (2008).
2007
Yang, J. C., Lessard, P. A. & Sinskey, A. J. Characterization of the mobilization determinants of pAN12, a small replicon from Rhodococcus erythropolis AN12. Plasmid 57, 71-81 (2007).
Yang, J. C. et al. TraA is required for megaplasmid conjugation in Rhodococcus erythropolis AN12. Plasmid 57, 55-70 (2007).
2006
Boccazzi, P. et al. Differential gene expression profiles and real-time measurements of growth parameters in Saccharomyces cerevisiae grown in microliter-scale bioreactors equipped with internal stirring. Biotechnol Prog 22, 710-7 (2006).

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