Publications

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2014
Grousseau, E., Lu, J., Gorret, N., Guillouet, S. E. & Sinskey, A. J. Isopropanol production with engineered Cupriavidus necator as bioproduction platform. Appl Microbiol Biotechnol 98, 4277-90 (2014).
2011
Bhubalan, K. et al. Characterization of the highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2. Appl Environ Microbiol 77, 2926-33 (2011).
Budde, C. F., Riedel, S. L., Willis, L. B., Rha, C. K. & Sinskey, A. J. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl Environ Microbiol 77, 2847-54 (2011).
2010
MacEachran, D. P., Prophete, M. E. & Sinskey, A. J. The Rhodococcus opacus PD630 heparin-binding hemagglutinin homolog TadA mediates lipid body formation. Appl Environ Microbiol 76, 7217-25 (2010).
2007
Yang, J. C., Lessard, P. A. & Sinskey, A. J. Characterization of the mobilization determinants of pAN12, a small replicon from Rhodococcus erythropolis AN12. Plasmid 57, 71-81 (2007).
Yang, J. C. et al. TraA is required for megaplasmid conjugation in Rhodococcus erythropolis AN12. Plasmid 57, 55-70 (2007).
2006
Kurosawa, K. et al. Characterization of Streptomyces MITKK-103, a newly isolated actinomycin X2-producer. Appl Microbiol Biotechnol 72, 145-154 (2006).
2005
Tian, J., Sinskey, A. J. & Stubbe, J. Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase. Biochemistry 44, 1495-503 (2005).
2003
York, G. M. et al. Ralstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genes. J Bacteriol 185, 3788-94 (2003).
2001
York, G. M., Stubbe, J. & Sinskey, A. J. New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate. J Bacteriol 183, 2394-7 (2001).
2000
Jia, Y., Kappock, T. J., Frick, T., Sinskey, A. J. & Stubbe, J. Lipases provide a new mechanistic model for polyhydroxybutyrate (PHB) synthases: characterization of the functional residues in Chromatium vinosum PHB synthase. Biochemistry 39, 3927-36 (2000).
1999
Lessard, P. A., O'Brien, X. M., Ahlgren, N. A., Ribich, S. A. & Sinskey, A. J. Characterization of IS1676 from Rhodococcus erythropolis SQ1. Appl Microbiol Biotechnol 52, 811-9 (1999).
Treadway, S. L. et al. Isolation and characterization of indene bioconversion genes from Rhodococcus strain I24. Appl Microbiol Biotechnol 51, 786-93 (1999).
Müh, U., Sinskey, A. J., Kirby, D. P., Lane, W. S. & Stubbe, J. PHA synthase from chromatium vinosum: cysteine 149 is involved in covalent catalysis. Biochemistry 38, 826-37 (1999).
1998
Koffas, M. A., Ramamoorthi, R., Pine, W. A., Sinskey, A. J. & Stephanopoulos, G. Sequence of the Corynebacterium glutamicum pyruvate carboxylase gene. Appl Microbiol Biotechnol 50, 346-52 (1998).
1995
Jetten, M. S., Follettie, M. T. & Sinskey, A. J. Effect of different levels of aspartokinase on the lysine production by Corynebacterium lactofermentum. Appl Microbiol Biotechnol 43, 76-82 (1995).
Jetten, M. S. & Sinskey, A. J. Recent advances in the physiology and genetics of amino acid-producing bacteria. Crit Rev Biotechnol 15, 73-103 (1995).
1994
Gubler, M., Jetten, M., Lee, S. H. & Sinskey, A. J. Cloning of the pyruvate kinase gene (pyk) of Corynebacterium glutamicum and site-specific inactivation of pyk in a lysine-producing Corynebacterium lactofermentum strain. Appl Environ Microbiol 60, 2494-500 (1994).
Shelikoff, M., Sinskey, A. J. & Stephanopoulos, G. The effect of protein synthesis inhibitors on the glycosylation site occupancy of recombinant human prolactin. Cytotechnology 15, 195-208 (1994).
Jetten, M. S., Follettie, M. T. & Sinskey, A. J. Metabolic engineering of Corynebacterium glutamicum. Ann N Y Acad Sci 721, 12-29 (1994).
Gerngross, T. U. et al. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry 33, 9311-20 (1994).
Jetten, M. S., Gubler, M. E., Lee, S. H. & Sinskey, A. J. Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum. Appl Environ Microbiol 60, 2501-7 (1994).
1993
Archer, J. A. & Sinskey, A. J. The DNA sequence and minimal replicon of the Corynebacterium glutamicum plasmid pSR1: evidence of a common ancestry with plasmids from C. diphtheriae. J Gen Microbiol 139, 1753-9 (1993).
Follettie, M. T., Peoples, O. P., Agoropoulou, C. & Sinskey, A. J. Gene structure and expression of the Corynebacterium flavum N13 ask-asd operon. J Bacteriol 175, 4096-103 (1993).
1992
Jain, J., Valge-Archer, V. E., Sinskey, A. J. & Rao, A. The AP-1 site at -150 bp, but not the NF-kappa B site, is likely to represent the major target of protein kinase C in the interleukin 2 promoter. J Exp Med 175, 853-62 (1992).
Williams, S. F. et al. Biosynthetic thiolase from Zoogloea ramigera. Mutagenesis of the putative active-site base Cys-378 to Ser-378 changes the partitioning of the acetyl S-enzyme intermediate. J Biol Chem 267, 16041-3 (1992).
Bor, Y. C. et al. Cloning and sequencing the Lactobacillus brevis gene encoding xylose isomerase. Gene 114, 127-32 (1992).
Liebl, W., Sinskey, A. J. & Schleifer, K. H. Expression, secretion, and processing of staphylococcal nuclease by Corynebacterium glutamicum. J Bacteriol 174, 1854-61 (1992).
Hwang, C., Sinskey, A. J. & Lodish, H. F. Oxidized redox state of glutathione in the endoplasmic reticulum. Science 257, 1496-502 (1992).
1991
Palmer, M. A. et al. Biosynthetic thiolase from Zoogloea ramigera. Evidence for a mechanism involving Cys-378 as the active site base. J Biol Chem 266, 8369-75 (1991).
Archer, J. A., Solow-Cordero, D. E. & Sinskey, A. J. A C-terminal deletion in Corynebacterium glutamicum homoserine dehydrogenase abolishes allosteric inhibition by L-threonine. Gene 107, 53-9 (1991).
1990
von der Osten, C. H. et al. Molecular cloning of aldolases for synthetic applications. Ann N Y Acad Sci 613, 771-5 (1990).
Han, K. S., Archer, J. A. & Sinskey, A. J. The molecular structure of the Corynebacterium glutamicum threonine synthase gene. Mol Microbiol 4, 1693-702 (1990).
Marcel, T., Archer, J. A., Mengin-Lecreulx, D. & Sinskey, A. J. Nucleotide sequence and organization of the upstream region of the Corynebacterium glutamicum lysA gene. Mol Microbiol 4, 1819-30 (1990).
1989
Peoples, O. P. & Sinskey, A. J. Fine structural analysis of the Zoogloea ramigera phbA-phbB locus encoding beta-ketothiolase and acetoacetyl-CoA reductase: nucleotide sequence of phbB. Mol Microbiol 3, 349-57 (1989).
von der Osten, C. H., Barbas, C. F., Wong, C. H. & Sinskey, A. J. Molecular cloning, nucleotide sequence and fine-structural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-1,6-biphosphate aldolase to class I and class II aldolases. Mol Microbiol 3, 1625-37 (1989).
Eikmanns, B. J., Follettie, M. T., Griot, M. U. & Sinskey, A. J. The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucleotide sequence, and expression. Mol Gen Genet 218, 330-9 (1989).
Peoples, O. P. & Sinskey, A. J. Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase. J Biol Chem 264, 15293-7 (1989).
Peoples, O. P. & Sinskey, A. J. Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J Biol Chem 264, 15298-303 (1989).
1988
Peoples, O. P. et al. Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom-thrB operon. Mol Microbiol 2, 63-72 (1988).
Yeh, P., Sicard, A. M. & Sinskey, A. J. Nucleotide sequence of the lysA gene of Corynebacterium glutamicum and possible mechanisms for modulation of its expression. Mol Gen Genet 212, 112-9 (1988).