Publications

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Journal Article
Juengert, J. R. et al. Absence of ppGpp Leads to Increased Mobilization of Intermediately Accumulated Poly(3-Hydroxybutyrate) in Ralstonia eutropha H16. Appl Environ Microbiol 83, (2017).
York, G. M., Junker, B. H., Stubbe, J. A. & Sinskey, A. J. Accumulation of the PhaP phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells. J Bacteriol 183, 4217-26 (2001).
Tian, J. et al. Analysis of transient polyhydroxybutyrate production in Wautersia eutropha H16 by quantitative Western analysis and transmission electron microscopy. J Bacteriol 187, 3825-32 (2005).
Wong, Y. - M., Brigham, C. J., Rha, C. K., Sinskey, A. J. & Sudesh, K. Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator. Bioresour Technol 121, 320-7 (2012).
Jeon, J. - M. et al. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha. Appl Microbiol Biotechnol 98, 5461-9 (2014).
Yang, Y. - H. et al. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) containing a predominant amount of 3-hydroxyvalerate by engineered Escherichia coli expressing propionate-CoA transferase. J Appl Microbiol 113, 815-23 (2012).
Lu, J., Brigham, C. J., Plassmeier, J. K. & Sinskey, A. J. Characterization and modification of enzymes in the 2-ketoisovalerate biosynthesis pathway of Ralstonia eutropha H16. Appl Microbiol Biotechnol 99, 761-74 (2015).
Lu, J., Brigham, C. J., Rha, C. K. & Sinskey, A. J. Characterization of an extracellular lipase and its chaperone from Ralstonia eutropha H16. Appl Microbiol Biotechnol 97, 2443-54 (2013).
Tian, J., Sinskey, A. J. & Stubbe, J. Class III polyhydroxybutyrate synthase: involvement in chain termination and reinitiation. Biochemistry 44, 8369-77 (2005).
Volova, T. G., Zhila, N. O., Kalacheva, G. S., Brigham, C. J. & Sinskey, A. J. Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha. Res Microbiol 164, 164-71 (2013).
Brigham, C. J. et al. Elucidation of beta-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression. J Bacteriol 192, 5454-64 (2010).
Bernardi, A. C., Gai, C. S., Lu, J., Sinskey, A. J. & Brigham, C. J. Experimental evolution and gene knockout studies reveal AcrA-mediated isobutanol tolerance in Ralstonia eutropha. J Biosci Bioeng 122, 64-9 (2016).
Budde, C. F. et al. Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium. Appl Microbiol Biotechnol 89, 1611-9 (2011).
Yang, Y. - H., Brigham, C., Willis, L., Rha, C. K. & Sinskey, A. Improved detergent-based recovery of polyhydroxyalkanoates (PHAs). Biotechnol Lett 33, 937-42 (2011).
Grousseau, E., Lu, J., Gorret, N., Guillouet, S. E. & Sinskey, A. J. Isopropanol production with engineered Cupriavidus necator as bioproduction platform. Appl Microbiol Biotechnol 98, 4277-90 (2014).
Grunwald, S. et al. Kinetic and stoichiometric characterization of organoautotrophic growth of Ralstonia eutropha on formic acid in fed-batch and continuous cultures. Microb Biotechnol 8, 155-63 (2015).
Tian, J., Sinskey, A. J. & Stubbe, J. Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy. J Bacteriol 187, 3814-24 (2005).
Brigham, C. J., Zhila, N., Shishatskaya, E., Volova, T. G. & Sinskey, A. J. Manipulation of Ralstonia eutropha carbon storage pathways to produce useful bio-based products. Subcell Biochem 64, 343-66 (2012).
Jia, Y. et al. Mechanistic studies on class I polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha: class I and III synthases share a similar catalytic mechanism. Biochemistry 40, 1011-9 (2001).
York, G. M., Stubbe, J. & Sinskey, A. J. New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate. J Bacteriol 183, 2394-7 (2001).
Yang, Y. - H. et al. Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. Appl Microbiol Biotechnol 87, 2037-45 (2010).
Marc, J. et al. Over expression of GroESL in Cupriavidus necator for heterotrophic and autotrophic isopropanol production. Metab Eng 42, 74-84 (2017).
Riedel, S. L. et al. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by Ralstonia eutropha in high cell density palm oil fermentations. Biotechnol Bioeng 109, 74-83 (2012).
Budde, C. F., Riedel, S. L., Willis, L. B., Rha, C. K. & Sinskey, A. J. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl Environ Microbiol 77, 2847-54 (2011).
Cho, M., Brigham, C. J., Sinskey, A. J. & Stubbe, J. Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo. Biochemistry 51, 2276-88 (2012).
York, G. M. et al. Ralstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genes. J Bacteriol 185, 3788-94 (2003).
York, G. M., Stubbe, J. & Sinskey, A. J. The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production. J Bacteriol 184, 59-66 (2002).
Riedel, S. L. et al. Recovery of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from Ralstonia eutropha cultures with non-halogenated solvents. Biotechnol Bioeng 110, 461-70 (2013).
Budde, C. F., Mahan, A. E., Lu, J., Rha, C. K. & Sinskey, A. J. Roles of multiple acetoacetyl coenzyme A reductases in polyhydroxybutyrate biosynthesis in Ralstonia eutropha H16. J Bacteriol 192, 5319-28 (2010).
Lu, J., Brigham, C. J., Gai, C. S. & Sinskey, A. J. Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha. Appl Microbiol Biotechnol 96, 283-97 (2012).
Volova, T. G., Zhila, N. O., Kalacheva, G. S., Sokolenko, V. A. & Sinskey, A. J. [Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria]. Prikl Biokhim Mikrobiol 47, 544-50 (2011).
Lawrence, A. G. et al. Transcriptional analysis of Ralstonia eutropha genes related to poly-(R)-3-hydroxybutyrate homeostasis during batch fermentation. Appl Microbiol Biotechnol 68, 663-72 (2005).
Brigham, C. J., Speth, D. R., Rha, C. K. & Sinskey, A. J. Whole-genome microarray and gene deletion studies reveal regulation of the polyhydroxyalkanoate production cycle by the stringent response in Ralstonia eutropha H16. Appl Environ Microbiol 78, 8033-44 (2012).