Publications

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Journal Article
York, G. M., Junker, B. H., Stubbe, J. A. & Sinskey, A. J. Accumulation of the PhaP phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells. J Bacteriol 183, 4217-26 (2001).
Jeon, J. - M. et al. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha. Appl Microbiol Biotechnol 98, 5461-9 (2014).
Bhubalan, K. et al. Characterization of the highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2. Appl Environ Microbiol 77, 2926-33 (2011).
Yuan, W. et al. Class I and III polyhydroxyalkanoate synthases from Ralstonia eutropha and Allochromatium vinosum: characterization and substrate specificity studies. Arch Biochem Biophys 394, 87-98 (2001).
Tian, J., Sinskey, A. J. & Stubbe, J. Class III polyhydroxybutyrate synthase: involvement in chain termination and reinitiation. Biochemistry 44, 8369-77 (2005).
Tian, J., Sinskey, A. J. & Stubbe, J. Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase. Biochemistry 44, 1495-503 (2005).
Peoples, O. P. & Sinskey, A. J. Fine structural analysis of the Zoogloea ramigera phbA-phbB locus encoding beta-ketothiolase and acetoacetyl-CoA reductase: nucleotide sequence of phbB. Mol Microbiol 3, 349-57 (1989).
Shaw-Reid, C. A., McCormick, M. M., Sinskey, A. J. & Stephanopoulos, G. Flux through the tetrahydrodipicolinate succinylase pathway is dispensable for L-lysine production in Corynebacterium glutamicum. Appl Microbiol Biotechnol 51, 325-33 (1999).
Gerngross, T. U., Reilly, P., Stubbe, J., Sinskey, A. J. & Peoples, O. P. Immunocytochemical analysis of poly-beta-hydroxybutyrate (PHB) synthase in Alcaligenes eutrophus H16: localization of the synthase enzyme at the surface of PHB granules. J Bacteriol 175, 5289-93 (1993).
Lawrence, A. G., Choi, J., Rha, C. K., Stubbe, J. & Sinskey, A. J. In vitro analysis of the chain termination reaction in the synthesis of poly-(R)-beta-hydroxybutyrate by the class III synthase from Allochromatium vinosum. Biomacromolecules 6, 2113-9 (2005).
Jia, Y., Kappock, T. J., Frick, T., Sinskey, A. J. & Stubbe, J. Lipases provide a new mechanistic model for polyhydroxybutyrate (PHB) synthases: characterization of the functional residues in Chromatium vinosum PHB synthase. Biochemistry 39, 3927-36 (2000).
Thompson, S. et al. Mechanistic studies on beta-ketoacyl thiolase from Zoogloea ramigera: identification of the active-site nucleophile as Cys89, its mutation to Ser89, and kinetic and thermodynamic characterization of wild-type and mutant enzymes. Biochemistry 28, 5735-42 (1989).
Jia, Y. et al. Mechanistic studies on class I polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha: class I and III synthases share a similar catalytic mechanism. Biochemistry 40, 1011-9 (2001).
Stubbe, J. et al. Nontemplate-dependent polymerization processes: polyhydroxyalkanoate synthases as a paradigm. Annu Rev Biochem 74, 433-80 (2005).
Gerngross, T. U. et al. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry 33, 9311-20 (1994).
Sim, S. J. et al. PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo. Nat Biotechnol 15, 63-7 (1997).
Müh, U., Sinskey, A. J., Kirby, D. P., Lane, W. S. & Stubbe, J. PHA synthase from chromatium vinosum: cysteine 149 is involved in covalent catalysis. Biochemistry 38, 826-37 (1999).
Peoples, O. P. & Sinskey, A. J. Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase. J Biol Chem 264, 15293-7 (1989).