Publications

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Filters: Keyword is Hydroxybutyrates  [Clear All Filters]
2017
Juengert, J. R. et al. Absence of ppGpp Leads to Increased Mobilization of Intermediately Accumulated Poly(3-Hydroxybutyrate) in Ralstonia eutropha H16. Appl Environ Microbiol 83, (2017).
2013
Volova, T. G., Zhila, N. O., Kalacheva, G. S., Brigham, C. J. & Sinskey, A. J. Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha. Res Microbiol 164, 164-71 (2013).
2012
Cho, M., Brigham, C. J., Sinskey, A. J. & Stubbe, J. Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo. Biochemistry 51, 2276-88 (2012).
Lu, J., Brigham, C. J., Gai, C. S. & Sinskey, A. J. Studies on the production of branched-chain alcohols in engineered Ralstonia eutropha. Appl Microbiol Biotechnol 96, 283-97 (2012).
2011
Budde, C. F. et al. Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium. Appl Microbiol Biotechnol 89, 1611-9 (2011).
Volova, T. G., Zhila, N. O., Kalacheva, G. S., Sokolenko, V. A. & Sinskey, A. J. [Synthesis of 3-hydroxybutyrate-CO-4-hydroxybutyrate copolymers by hydrogen-oxidizing bacteria]. Prikl Biokhim Mikrobiol 47, 544-50 (2011).
2010
Brigham, C. J. et al. Elucidation of beta-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression. J Bacteriol 192, 5454-64 (2010).
Budde, C. F., Mahan, A. E., Lu, J., Rha, C. K. & Sinskey, A. J. Roles of multiple acetoacetyl coenzyme A reductases in polyhydroxybutyrate biosynthesis in Ralstonia eutropha H16. J Bacteriol 192, 5319-28 (2010).
2005
Tian, J. et al. Analysis of transient polyhydroxybutyrate production in Wautersia eutropha H16 by quantitative Western analysis and transmission electron microscopy. J Bacteriol 187, 3825-32 (2005).
Tian, J., Sinskey, A. J. & Stubbe, J. Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase. Biochemistry 44, 1495-503 (2005).
Lawrence, A. G., Choi, J., Rha, C. K., Stubbe, J. & Sinskey, A. J. In vitro analysis of the chain termination reaction in the synthesis of poly-(R)-beta-hydroxybutyrate by the class III synthase from Allochromatium vinosum. Biomacromolecules 6, 2113-9 (2005).
Tian, J., Sinskey, A. J. & Stubbe, J. Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy. J Bacteriol 187, 3814-24 (2005).
Lawrence, A. G. et al. Transcriptional analysis of Ralstonia eutropha genes related to poly-(R)-3-hydroxybutyrate homeostasis during batch fermentation. Appl Microbiol Biotechnol 68, 663-72 (2005).
2003
York, G. M. et al. Ralstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genes. J Bacteriol 185, 3788-94 (2003).
2002
York, G. M., Stubbe, J. & Sinskey, A. J. The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production. J Bacteriol 184, 59-66 (2002).
2001
York, G. M., Junker, B. H., Stubbe, J. A. & Sinskey, A. J. Accumulation of the PhaP phasin of Ralstonia eutropha is dependent on production of polyhydroxybutyrate in cells. J Bacteriol 183, 4217-26 (2001).
York, G. M., Stubbe, J. & Sinskey, A. J. New insight into the role of the PhaP phasin of Ralstonia eutropha in promoting synthesis of polyhydroxybutyrate. J Bacteriol 183, 2394-7 (2001).
1999
Müh, U., Sinskey, A. J., Kirby, D. P., Lane, W. S. & Stubbe, J. PHA synthase from chromatium vinosum: cysteine 149 is involved in covalent catalysis. Biochemistry 38, 826-37 (1999).
1997
Sim, S. J. et al. PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo. Nat Biotechnol 15, 63-7 (1997).
1989
Peoples, O. P. & Sinskey, A. J. Fine structural analysis of the Zoogloea ramigera phbA-phbB locus encoding beta-ketothiolase and acetoacetyl-CoA reductase: nucleotide sequence of phbB. Mol Microbiol 3, 349-57 (1989).
Peoples, O. P. & Sinskey, A. J. Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase. J Biol Chem 264, 15293-7 (1989).
Peoples, O. P. & Sinskey, A. J. Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J Biol Chem 264, 15298-303 (1989).