Publications

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2016
Bernardi, A. C., Gai, C. S., Lu, J., Sinskey, A. J. & Brigham, C. J. Experimental evolution and gene knockout studies reveal AcrA-mediated isobutanol tolerance in Ralstonia eutropha. J Biosci Bioeng 122, 64-9 (2016).
2012
Yang, Y. - H. et al. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) containing a predominant amount of 3-hydroxyvalerate by engineered Escherichia coli expressing propionate-CoA transferase. J Appl Microbiol 113, 815-23 (2012).
Cho, M., Brigham, C. J., Sinskey, A. J. & Stubbe, J. Purification of polyhydroxybutyrate synthase from its native organism, Ralstonia eutropha: implications for the initiation and elongation of polymer formation in vivo. Biochemistry 51, 2276-88 (2012).
2011
Bhubalan, K. et al. Characterization of the highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2. Appl Environ Microbiol 77, 2926-33 (2011).
Yang, Y. - H., Brigham, C., Willis, L., Rha, C. K. & Sinskey, A. Improved detergent-based recovery of polyhydroxyalkanoates (PHAs). Biotechnol Lett 33, 937-42 (2011).
2006
Zanzotto, A. et al. In situ measurement of bioluminescence and fluorescence in an integrated microbioreactor. Biotechnol Bioeng 93, 40-7 (2006).
Lee, H. L. T., Boccazzi, P., Ram, R. J. & Sinskey, A. J. Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control. Lab Chip 6, 1229-35 (2006).
Zhang, Z., Szita, N., Boccazzi, P., Sinskey, A. J. & Jensen, K. F. A well-mixed, polymer-based microbioreactor with integrated optical measurements. Biotechnol Bioeng 93, 286-96 (2006).
2005
Szita, N. et al. Development of a multiplexed microbioreactor system for high-throughput bioprocessing. Lab Chip 5, 819-26 (2005).
Boccazzi, P. et al. Gene expression analysis of Escherichia coli grown in miniaturized bioreactor platforms for high-throughput analysis of growth and genomic data. Appl Microbiol Biotechnol 68, 518-32 (2005).
Lawrence, A. G., Choi, J., Rha, C. K., Stubbe, J. & Sinskey, A. J. In vitro analysis of the chain termination reaction in the synthesis of poly-(R)-beta-hydroxybutyrate by the class III synthase from Allochromatium vinosum. Biomacromolecules 6, 2113-9 (2005).
2004
Zanzotto, A. et al. Membrane-aerated microbioreactor for high-throughput bioprocessing. Biotechnol Bioeng 87, 243-54 (2004).
2002
York, G. M., Stubbe, J. & Sinskey, A. J. The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production. J Bacteriol 184, 59-66 (2002).
1999
Guillouet, S., Rodal, A. A., An, G., Lessard, P. A. & Sinskey, A. J. Expression of the Escherichia coli catabolic threonine dehydratase in Corynebacterium glutamicum and its effect on isoleucine production. Appl Environ Microbiol 65, 3100-7 (1999).
Park, S. M., Klapa, M. I., Sinskey, A. J. & Stephanopoulos, G. Metabolite and isotopomer balancing in the analysis of metabolic cycles: II. Applications. Biotechnol Bioeng 62, 392-401 (1999).
1997
Sim, S. J. et al. PHA synthase activity controls the molecular weight and polydispersity of polyhydroxybutyrate in vivo. Nat Biotechnol 15, 63-7 (1997).
1994
Gerngross, T. U. et al. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry 33, 9311-20 (1994).
1993
Follettie, M. T., Peoples, O. P., Agoropoulou, C. & Sinskey, A. J. Gene structure and expression of the Corynebacterium flavum N13 ask-asd operon. J Bacteriol 175, 4096-103 (1993).
1990
von der Osten, C. H. et al. Molecular cloning of aldolases for synthetic applications. Ann N Y Acad Sci 613, 771-5 (1990).
Han, K. S., Archer, J. A. & Sinskey, A. J. The molecular structure of the Corynebacterium glutamicum threonine synthase gene. Mol Microbiol 4, 1693-702 (1990).
Marcel, T., Archer, J. A., Mengin-Lecreulx, D. & Sinskey, A. J. Nucleotide sequence and organization of the upstream region of the Corynebacterium glutamicum lysA gene. Mol Microbiol 4, 1819-30 (1990).
1989
Peoples, O. P. & Sinskey, A. J. Fine structural analysis of the Zoogloea ramigera phbA-phbB locus encoding beta-ketothiolase and acetoacetyl-CoA reductase: nucleotide sequence of phbB. Mol Microbiol 3, 349-57 (1989).
Peoples, O. P. & Sinskey, A. J. Poly-beta-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16. Characterization of the genes encoding beta-ketothiolase and acetoacetyl-CoA reductase. J Biol Chem 264, 15293-7 (1989).
Peoples, O. P. & Sinskey, A. J. Poly-beta-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16. Identification and characterization of the PHB polymerase gene (phbC). J Biol Chem 264, 15298-303 (1989).
1988
Yeh, P., Sicard, A. M. & Sinskey, A. J. General organization of the genes specifically involved in the diaminopimelate-lysine biosynthetic pathway of Corynebacterium glutamicum. Mol Gen Genet 212, 105-11 (1988).
Yeh, P., Sicard, A. M. & Sinskey, A. J. Nucleotide sequence of the lysA gene of Corynebacterium glutamicum and possible mechanisms for modulation of its expression. Mol Gen Genet 212, 112-9 (1988).
1987
Peoples, O. P., Masamune, S., Walsh, C. T. & Sinskey, A. J. Biosynthetic thiolase from Zoogloea ramigera. III. Isolation and characterization of the structural gene. J Biol Chem 262, 97-102 (1987).
1986
Follettie, M. T. & Sinskey, A. J. Molecular cloning and nucleotide sequence of the Corynebacterium glutamicum pheA gene. J Bacteriol 167, 695-702 (1986).
1983
Pellon, J. R., Sinskey, A. J., Hecht, S. M. & Gomez, R. F. Kinetics of the bleomycin A2 damage in vivo to the folded chromosome of Escherichia coli. Chem Biol Interact 43, 245-51 (1983).
1979
Ulmer, K. M., Gomez, R. F. & Sinskey, A. J. Ionizing radiation damage to the folded chromosome of Escherichia coli K-12: repair of double-strand breaks in deoxyribonucleic acid. J Bacteriol 138, 486-91 (1979).
Ulmer, K. M., Gomez, R. F. & Sinskey, A. J. Ionizing radiation damage to the folded chromosome of Escherichia coli K-12: sedimentation properties of irradiated nucleoids and chromosomal deoxyribonucleic acid. J Bacteriol 138, 475-85 (1979).
1973
Gomez, R., Takano, M. & Sinskey, A. J. Proceedings: Characteristics of freeze-dried cells. Cryobiology 10, 368-74 (1973).