Publications

Export 5 results:
Author Title Type [ Year(Desc)]
Filters: Keyword is Mutagenesis, Site-Directed  [Clear All Filters]
1992
Williams, S. F. et al. Biosynthetic thiolase from Zoogloea ramigera. Mutagenesis of the putative active-site base Cys-378 to Ser-378 changes the partitioning of the acetyl S-enzyme intermediate. J Biol Chem 267, 16041-3 (1992).
1994
Gerngross, T. U. et al. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus: evidence for a required posttranslational modification for catalytic activity. Biochemistry 33, 9311-20 (1994).
1999
Müh, U., Sinskey, A. J., Kirby, D. P., Lane, W. S. & Stubbe, J. PHA synthase from chromatium vinosum: cysteine 149 is involved in covalent catalysis. Biochemistry 38, 826-37 (1999).
2005
Tian, J., Sinskey, A. J. & Stubbe, J. Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase. Biochemistry 44, 1495-503 (2005).
2015
Lu, J., Brigham, C. J., Plassmeier, J. K. & Sinskey, A. J. Characterization and modification of enzymes in the 2-ketoisovalerate biosynthesis pathway of Ralstonia eutropha H16. Appl Microbiol Biotechnol 99, 761-74 (2015).