Publications

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Farrar, C. T. et al. 3D molecular MR imaging of liver fibrosis and response to rapamycin therapy in a bile duct ligation rat model. J Hepatol 63, 689-96 (2015).
A
Juengert, J. R. et al. Absence of ppGpp Leads to Increased Mobilization of Intermediately Accumulated Poly(3-Hydroxybutyrate) in Ralstonia eutropha H16. Appl Environ Microbiol 83, (2017).
Tian, J. et al. Analysis of transient polyhydroxybutyrate production in Wautersia eutropha H16 by quantitative Western analysis and transmission electron microscopy. J Bacteriol 187, 3825-32 (2005).
Kurosawa, K., MacEachran, D. P. & Sinskey, A. J. Antibiotic biosynthesis following horizontal gene transfer: new milestone for novel natural product discovery?. Expert Opin Drug Discov 5, 819-25 (2010).
Miyasaka, Y., Rha, C. K. & Sinskey, A. J. Application of temperature-sensitive mutants for single-cell protein production. Biotechnol Bioeng 22, 2065-2079 (1980).
Rafat, M. et al. Association (micellization) and partitioning of aglycon triterpenoids. J Colloid Interface Sci 325, 324-30 (2008).
B
Gorret, N. et al. Bioreactor culture of oil palm (Elaeis guineensis) and effects of nitrogen source, inoculum size, and conditioned medium on biomass production. J Biotechnol 108, 253-63 (2004).
Wong, Y. - M., Brigham, C. J., Rha, C. K., Sinskey, A. J. & Sudesh, K. Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator. Bioresour Technol 121, 320-7 (2012).
Jeon, J. - M. et al. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(HB-co-HHx)) from butyrate using engineered Ralstonia eutropha. Appl Microbiol Biotechnol 98, 5461-9 (2014).
C
Lu, J., Brigham, C. J., Plassmeier, J. K. & Sinskey, A. J. Characterization and modification of enzymes in the 2-ketoisovalerate biosynthesis pathway of Ralstonia eutropha H16. Appl Microbiol Biotechnol 99, 761-74 (2015).
Lu, J., Brigham, C. J., Rha, C. K. & Sinskey, A. J. Characterization of an extracellular lipase and its chaperone from Ralstonia eutropha H16. Appl Microbiol Biotechnol 97, 2443-54 (2013).
Bhubalan, K. et al. Characterization of the highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2. Appl Environ Microbiol 77, 2926-33 (2011).
Yang, J. C., Lessard, P. A. & Sinskey, A. J. Characterization of the mobilization determinants of pAN12, a small replicon from Rhodococcus erythropolis AN12. Plasmid 57, 71-81 (2007).
Tian, J., Sinskey, A. J. & Stubbe, J. Class III polyhydroxybutyrate synthase: involvement in chain termination and reinitiation. Biochemistry 44, 8369-77 (2005).
Holder, J. W. et al. Comparative and functional genomics of Rhodococcus opacus PD630 for biofuels development. PLoS Genet 7, e1002219 (2011).
Brigham, C. J., Speth, D. R., Rha, C. K. & Sinskey, A. J. Correction for Brigham et al., "Whole-Genome Microarray and Gene Deletion Studies Reveal Regulation of the Polyhydroxyalkanoate Production Cycle by the Stringent Response in Ralstonia eutropha H16". Appl Environ Microbiol 83, (2017).
Bernardi, A. C., Gai, C. S., Lu, J., Sinskey, A. J. & Brigham, C. J. Corrigendum to "Experimental evolution and gene knockout studies reveal AcrA-mediated isobutanol tolerance in Ralstonia eutropha" [J Biosci Bioeng 122 (2016) 64-69]. J Biosci Bioeng 123, 658 (2017).
D
Tian, J., Sinskey, A. J. & Stubbe, J. Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase. Biochemistry 44, 1495-503 (2005).
Szita, N. et al. Development of a multiplexed microbioreactor system for high-throughput bioprocessing. Lab Chip 5, 819-26 (2005).
Boccazzi, P. et al. Differential gene expression profiles and real-time measurements of growth parameters in Saccharomyces cerevisiae grown in microliter-scale bioreactors equipped with internal stirring. Biotechnol Prog 22, 710-7 (2006).
E
Volova, T. G., Zhila, N. O., Kalacheva, G. S., Brigham, C. J. & Sinskey, A. J. Effects of intracellular poly(3-hydroxybutyrate) reserves on physiological-biochemical properties and growth of Ralstonia eutropha. Res Microbiol 164, 164-71 (2013).
Brigham, C. J. et al. Elucidation of beta-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression. J Bacteriol 192, 5454-64 (2010).
Song, Y. et al. Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production. Appl Microbiol Biotechnol 93, 1917-25 (2012).
Kurosawa, K., Plassmeier, J., Kalinowski, J., Rückert, C. & Sinskey, A. J. Engineering L-arabinose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Metab Eng 30, 89-95 (2015).
Kurosawa, K., Wewetzer, S. J. & Sinskey, A. J. Engineering xylose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Biotechnol Biofuels 6, 134 (2013).
Brigham, C. J., Reimer, E. N., Rha, C. K. & Sinskey, A. J. Examination of PHB Depolymerases in Ralstonia eutropha: Further Elucidation of the Roles of Enzymes in PHB Homeostasis. AMB Express 2, 26 (2012).
Bernardi, A. C., Gai, C. S., Lu, J., Sinskey, A. J. & Brigham, C. J. Experimental evolution and gene knockout studies reveal AcrA-mediated isobutanol tolerance in Ralstonia eutropha. J Biosci Bioeng 122, 64-9 (2016).
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Stallings, S. C. et al. A framework to evaluate the economic impact of pharmacogenomics. Pharmacogenomics 7, 853-62 (2006).
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Boccazzi, P. et al. Gene expression analysis of Escherichia coli grown in miniaturized bioreactor platforms for high-throughput analysis of growth and genomic data. Appl Microbiol Biotechnol 68, 518-32 (2005).
Coldren, C. D. et al. Gene expression changes in the human fibroblast induced by Centella asiatica triterpenoids. Planta Med 69, 725-32 (2003).
Osman, A. et al. Genetic diversity of Eurycoma longifolia inferred from single nucleotide polymorphisms. Plant Physiol 131, 1294-301 (2003).
Budde, C. F. et al. Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium. Appl Microbiol Biotechnol 89, 1611-9 (2011).
H
Kurosawa, K., Boccazzi, P., de Almeida, N. M. & Sinskey, A. J. High-cell-density batch fermentation of Rhodococcus opacus PD630 using a high glucose concentration for triacylglycerol production. J Biotechnol 147, 212-8 (2010).
I
Kurosawa, K., Radek, A., Plassmeier, J. K. & Sinskey, A. J. Improved glycerol utilization by a triacylglycerol-producing Rhodococcus opacus strain for renewable fuels. Biotechnol Biofuels 8, 31 (2015).
Zanzotto, A. et al. In situ measurement of bioluminescence and fluorescence in an integrated microbioreactor. Biotechnol Bioeng 93, 40-7 (2006).
Lawrence, A. G., Choi, J., Rha, C. K., Stubbe, J. & Sinskey, A. J. In vitro analysis of the chain termination reaction in the synthesis of poly-(R)-beta-hydroxybutyrate by the class III synthase from Allochromatium vinosum. Biomacromolecules 6, 2113-9 (2005).
Priefert, H. et al. Indene bioconversion by a toluene inducible dioxygenase of Rhodococcus sp. I24. Appl Microbiol Biotechnol 65, 168-76 (2004).
Gai, C. S., Lu, J., Brigham, C. J., Bernardi, A. C. & Sinskey, A. J. Insights into bacterial CO2 metabolism revealed by the characterization of four carbonic anhydrases in Ralstonia eutropha H16. AMB Express 4, 2 (2014).
Grousseau, E., Lu, J., Gorret, N., Guillouet, S. E. & Sinskey, A. J. Isopropanol production with engineered Cupriavidus necator as bioproduction platform. Appl Microbiol Biotechnol 98, 4277-90 (2014).
K
Tian, J., Sinskey, A. J. & Stubbe, J. Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy. J Bacteriol 187, 3814-24 (2005).
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Kurosawa, K., C Debono, A. & Sinskey, A. J. Lignocellulose-derived inhibitors improve lipid extraction from wet Rhodococcus opacus cells. Bioresour Technol 193, 206-12 (2015).
M
Lessard, P. A., Kulaveerasingam, H., York, G. M., Strong, A. & Sinskey, A. J. Manipulating gene expression for the metabolic engineering of plants. Metab Eng 4, 67-79 (2002).
Brigham, C. J., Zhila, N., Shishatskaya, E., Volova, T. G. & Sinskey, A. J. Manipulation of Ralstonia eutropha carbon storage pathways to produce useful bio-based products. Subcell Biochem 64, 343-66 (2012).
Zanzotto, A. et al. Membrane-aerated microbioreactor for high-throughput bioprocessing. Biotechnol Bioeng 87, 243-54 (2004).
Plassmeier, J., Li, Y., Rueckert, C. & Sinskey, A. J. Metabolic engineering Corynebacterium glutamicum to produce triacylglycerols. Metab Eng 33, 86-97 (2016).
Lee, H. L. T., Boccazzi, P., Ram, R. J. & Sinskey, A. J. Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control. Lab Chip 6, 1229-35 (2006).
Zhang, Z. et al. Microchemostat-microbial continuous culture in a polymer-based, instrumented microbioreactor. Lab Chip 6, 906-13 (2006).
Lee, K. S., Boccazzi, P., Sinskey, A. J. & Ram, R. J. Microfluidic chemostat and turbidostat with flow rate, oxygen, and temperature control for dynamic continuous culture. Lab Chip 11, 1730-9 (2011).
Fuchs, B. C. et al. Molecular MRI of collagen to diagnose and stage liver fibrosis. J Hepatol 59, 992-8 (2013).
N
Stubbe, J. et al. Nontemplate-dependent polymerization processes: polyhydroxyalkanoate synthases as a paradigm. Annu Rev Biochem 74, 433-80 (2005).

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