Publications

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1980
Miyasaka, Y., Rha, C. K. & Sinskey, A. J. Application of temperature-sensitive mutants for single-cell protein production. Biotechnol Bioeng 22, 2065-2079 (1980).
2002
Lessard, P. A., Kulaveerasingam, H., York, G. M., Strong, A. & Sinskey, A. J. Manipulating gene expression for the metabolic engineering of plants. Metab Eng 4, 67-79 (2002).
Stafford, D. E. et al. Optimizing bioconversion pathways through systems analysis and metabolic engineering. Proc Natl Acad Sci U S A 99, 1801-6 (2002).
York, G. M., Stubbe, J. & Sinskey, A. J. The Ralstonia eutropha PhaR protein couples synthesis of the PhaP phasin to the presence of polyhydroxybutyrate in cells and promotes polyhydroxybutyrate production. J Bacteriol 184, 59-66 (2002).
2003
Coldren, C. D. et al. Gene expression changes in the human fibroblast induced by Centella asiatica triterpenoids. Planta Med 69, 725-32 (2003).
Osman, A. et al. Genetic diversity of Eurycoma longifolia inferred from single nucleotide polymorphisms. Plant Physiol 131, 1294-301 (2003).
Nielsen, U. B., Cardone, M. H., Sinskey, A. J., MacBeath, G. & Sorger, P. K. Profiling receptor tyrosine kinase activation by using Ab microarrays. Proc Natl Acad Sci U S A 100, 9330-5 (2003).
York, G. M. et al. Ralstonia eutropha H16 encodes two and possibly three intracellular Poly[D-(-)-3-hydroxybutyrate] depolymerase genes. J Bacteriol 185, 3788-94 (2003).
2004
Gorret, N. et al. Bioreactor culture of oil palm (Elaeis guineensis) and effects of nitrogen source, inoculum size, and conditioned medium on biomass production. J Biotechnol 108, 253-63 (2004).
Priefert, H. et al. Indene bioconversion by a toluene inducible dioxygenase of Rhodococcus sp. I24. Appl Microbiol Biotechnol 65, 168-76 (2004).
Zanzotto, A. et al. Membrane-aerated microbioreactor for high-throughput bioprocessing. Biotechnol Bioeng 87, 243-54 (2004).
Lessard, P. A., O'Brien, X. M., Currie, D. H. & Sinskey, A. J. pB264, a small, mobilizable, temperature sensitive plasmid from Rhodococcus. BMC Microbiol 4, 15 (2004).
Ozminkowski, R. J. et al. The use of disease-modifying new drugs for multiple sclerosis treatment in private-sector health plans. Clin Ther 26, 1341-54 (2004).
2005
Tian, J. et al. Analysis of transient polyhydroxybutyrate production in Wautersia eutropha H16 by quantitative Western analysis and transmission electron microscopy. J Bacteriol 187, 3825-32 (2005).
Tian, J., Sinskey, A. J. & Stubbe, J. Class III polyhydroxybutyrate synthase: involvement in chain termination and reinitiation. Biochemistry 44, 8369-77 (2005).
Tian, J., Sinskey, A. J. & Stubbe, J. Detection of intermediates from the polymerization reaction catalyzed by a D302A mutant of class III polyhydroxyalkanoate (PHA) synthase. Biochemistry 44, 1495-503 (2005).
Szita, N. et al. Development of a multiplexed microbioreactor system for high-throughput bioprocessing. Lab Chip 5, 819-26 (2005).
Boccazzi, P. et al. Gene expression analysis of Escherichia coli grown in miniaturized bioreactor platforms for high-throughput analysis of growth and genomic data. Appl Microbiol Biotechnol 68, 518-32 (2005).
Lawrence, A. G., Choi, J., Rha, C. K., Stubbe, J. & Sinskey, A. J. In vitro analysis of the chain termination reaction in the synthesis of poly-(R)-beta-hydroxybutyrate by the class III synthase from Allochromatium vinosum. Biomacromolecules 6, 2113-9 (2005).
Tian, J., Sinskey, A. J. & Stubbe, J. Kinetic studies of polyhydroxybutyrate granule formation in Wautersia eutropha H16 by transmission electron microscopy. J Bacteriol 187, 3814-24 (2005).
Stubbe, J. et al. Nontemplate-dependent polymerization processes: polyhydroxyalkanoate synthases as a paradigm. Annu Rev Biochem 74, 433-80 (2005).
Lawrence, A. G. et al. Transcriptional analysis of Ralstonia eutropha genes related to poly-(R)-3-hydroxybutyrate homeostasis during batch fermentation. Appl Microbiol Biotechnol 68, 663-72 (2005).
2006
Boccazzi, P. et al. Differential gene expression profiles and real-time measurements of growth parameters in Saccharomyces cerevisiae grown in microliter-scale bioreactors equipped with internal stirring. Biotechnol Prog 22, 710-7 (2006).
Stallings, S. C. et al. A framework to evaluate the economic impact of pharmacogenomics. Pharmacogenomics 7, 853-62 (2006).
Zanzotto, A. et al. In situ measurement of bioluminescence and fluorescence in an integrated microbioreactor. Biotechnol Bioeng 93, 40-7 (2006).
Lee, H. L. T., Boccazzi, P., Ram, R. J. & Sinskey, A. J. Microbioreactor arrays with integrated mixers and fluid injectors for high-throughput experimentation with pH and dissolved oxygen control. Lab Chip 6, 1229-35 (2006).
Zhang, Z. et al. Microchemostat-microbial continuous culture in a polymer-based, instrumented microbioreactor. Lab Chip 6, 906-13 (2006).
Plaggenborg, R. et al. Potential of Rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol. Appl Microbiol Biotechnol 72, 745-55 (2006).
Zhang, Z., Szita, N., Boccazzi, P., Sinskey, A. J. & Jensen, K. F. A well-mixed, polymer-based microbioreactor with integrated optical measurements. Biotechnol Bioeng 93, 286-96 (2006).
2007
Yang, J. C., Lessard, P. A. & Sinskey, A. J. Characterization of the mobilization determinants of pAN12, a small replicon from Rhodococcus erythropolis AN12. Plasmid 57, 71-81 (2007).
Yang, J. C. et al. TraA is required for megaplasmid conjugation in Rhodococcus erythropolis AN12. Plasmid 57, 55-70 (2007).
2008
Rafat, M. et al. Association (micellization) and partitioning of aglycon triterpenoids. J Colloid Interface Sci 325, 324-30 (2008).
Kurosawa, K. et al. Rhodostreptomycins, antibiotics biosynthesized following horizontal gene transfer from Streptomyces padanus to Rhodococcus fascians. J Am Chem Soc 130, 1126-7 (2008).
2010
Kurosawa, K., MacEachran, D. P. & Sinskey, A. J. Antibiotic biosynthesis following horizontal gene transfer: new milestone for novel natural product discovery?. Expert Opin Drug Discov 5, 819-25 (2010).
Brigham, C. J. et al. Elucidation of beta-oxidation pathways in Ralstonia eutropha H16 by examination of global gene expression. J Bacteriol 192, 5454-64 (2010).
Kurosawa, K., Boccazzi, P., de Almeida, N. M. & Sinskey, A. J. High-cell-density batch fermentation of Rhodococcus opacus PD630 using a high glucose concentration for triacylglycerol production. J Biotechnol 147, 212-8 (2010).
Yang, Y. - H. et al. Optimization of growth media components for polyhydroxyalkanoate (PHA) production from organic acids by Ralstonia eutropha. Appl Microbiol Biotechnol 87, 2037-45 (2010).
Budde, C. F., Mahan, A. E., Lu, J., Rha, C. K. & Sinskey, A. J. Roles of multiple acetoacetyl coenzyme A reductases in polyhydroxybutyrate biosynthesis in Ralstonia eutropha H16. J Bacteriol 192, 5319-28 (2010).
Puglisi, E. et al. Transcriptional response of Rhodococcus aetherivorans I24 to polychlorinated biphenyl-contaminated sediments. Microb Ecol 60, 505-15 (2010).
2011
Bhubalan, K. et al. Characterization of the highly active polyhydroxyalkanoate synthase of Chromobacterium sp. strain USM2. Appl Environ Microbiol 77, 2926-33 (2011).
Holder, J. W. et al. Comparative and functional genomics of Rhodococcus opacus PD630 for biofuels development. PLoS Genet 7, e1002219 (2011).
Budde, C. F. et al. Growth and polyhydroxybutyrate production by Ralstonia eutropha in emulsified plant oil medium. Appl Microbiol Biotechnol 89, 1611-9 (2011).
Lee, K. S., Boccazzi, P., Sinskey, A. J. & Ram, R. J. Microfluidic chemostat and turbidostat with flow rate, oxygen, and temperature control for dynamic continuous culture. Lab Chip 11, 1730-9 (2011).
Sambanthamurthi, R. et al. Oil palm vegetation liquor: a new source of phenolic bioactives. Br J Nutr 106, 1655-63 (2011).
Sambanthamurthi, R. et al. Positive outcomes of oil palm phenolics on degenerative diseases in animal models. Br J Nutr 106, 1664-75 (2011).
Budde, C. F., Riedel, S. L., Willis, L. B., Rha, C. K. & Sinskey, A. J. Production of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from plant oil by engineered Ralstonia eutropha strains. Appl Environ Microbiol 77, 2847-54 (2011).
2012
Wong, Y. - M., Brigham, C. J., Rha, C. K., Sinskey, A. J. & Sudesh, K. Biosynthesis and characterization of polyhydroxyalkanoate containing high 3-hydroxyhexanoate monomer fraction from crude palm kernel oil by recombinant Cupriavidus necator. Bioresour Technol 121, 320-7 (2012).
Song, Y. et al. Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production. Appl Microbiol Biotechnol 93, 1917-25 (2012).
Brigham, C. J., Reimer, E. N., Rha, C. K. & Sinskey, A. J. Examination of PHB Depolymerases in Ralstonia eutropha: Further Elucidation of the Roles of Enzymes in PHB Homeostasis. AMB Express 2, 26 (2012).
Brigham, C. J., Zhila, N., Shishatskaya, E., Volova, T. G. & Sinskey, A. J. Manipulation of Ralstonia eutropha carbon storage pathways to produce useful bio-based products. Subcell Biochem 64, 343-66 (2012).

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