Recent advances in the physiology and genetics of amino acid-producing bacteria.

TitleRecent advances in the physiology and genetics of amino acid-producing bacteria.
Publication TypeJournal Article
Year of Publication1995
AuthorsJetten, MS, Sinskey, AJ
JournalCrit Rev Biotechnol
Date Published1995
KeywordsAmino Acids, Base Sequence, Cloning, Molecular, Corynebacterium, DNA, Bacterial, Gene Expression Regulation, Bacterial, Genes, Bacterial, Genetic Engineering, Glutamic Acid, Isoleucine, Lysine, Molecular Sequence Data, Threonine

Corynebacterium glutamicum and its close relatives, C. flavum and C. lactofermentum, have been used for over 3 decades in the industrial production of amino acids by fermentation. Since 1984, several research groups have started programs to develop metabolic engineering principles for amino acid-producing Corynebacterium strains. Initially, the programs concentrated on the isolation of genes encoding (deregulated) biosynthetic enzymes and the development of general molecular biology tools such as cloning vectors and DNA transfer methods. With most of the genes and tools now available, recombinant DNA technology can be applied in strain improvement. To accomplish these improvements, it is critical and advantageous to understand the mechanisms of gene expression and regulation as well as the biochemistry and physiology of the species being engineered. This review explores the advances made in the understanding and application of amino acid-producing bacteria in the early 1990s.

Alternate JournalCrit Rev Biotechnol
Citation Key254
PubMed ID7736600