Transformation of T lymphocytes by the v-fos oncogene.

TitleTransformation of T lymphocytes by the v-fos oncogene.
Publication TypeJournal Article
Year of Publication1990
AuthorsValge-Archer, VE, de Villiers, J, Sinskey, AJ, Rao, A
JournalJ Immunol
Date Published1990 Dec 15
KeywordsAnimals, Blotting, Southern, Cell Line, Cell Transformation, Viral, Gene Expression, In Vitro Techniques, Interleukin-2, Lymphocyte Activation, Mice, Oncogene Proteins v-fos, Oncogene Proteins, Viral, Oncogenes, Plasmids, RNA, Messenger, T-Lymphocytes

Activation of T lymphocytes through the T cell antigen receptor has been shown to stimulate a rapid and transient accumulation of c-fos mRNA and protein. Transfection of a normal murine T lymphocyte clone with the FBJ-v-fos oncogene resulted in generation of a cell line that was morphologically transformed, had lost the requirement for IL-2 for proliferation, and was tumorigenic in adult syngeneic mice; however, the transformed cells retained the ability to proliferate in response to IL-2. The transformed cells did not show constitutive expression of IL-2 or c-fos mRNA, although the promoter regions of both IL-2 and c-fos genes contain AP-1 sites that are expected to be targets for binding of Fos/Jun complexes. In contrast, the transformed T cells showed increased constitutive expression of IL-2R alpha and c-myc mRNA; these genes may represent cellular targets for transformation by v-fos and physiologic activation by c-fos. We discuss the possibility that these transformed cells behave as cells partially activated through the TCR, and that transformation occurs through a mechanism independent of IL-2.

Alternate JournalJ Immunol
Citation Key154
PubMed ID2124241
Grant ListCA42471 / CA / NCI NIH HHS / United States